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Related Concept Videos

Electron Transport Chain Components01:29

Electron Transport Chain Components

1.2K
The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...
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Electron Transport Chains01:28

Electron Transport Chains

115.5K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
115.5K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

9.6K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
9.6K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

19.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.5K
The Electron Transport Chain01:30

The Electron Transport Chain

21.2K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
21.2K
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

3.1K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
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Related Experiment Video

Updated: Mar 21, 2026

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
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Bacterial Electron Transfer Chains Primed by Proteomics.

H J C T Wessels1, N M de Almeida2, B Kartal3

  • 1Nijmegen Center for Mitochondrial Disorders, Radboud Proteomics Centre, Translational Metabolic Laboratory, Radboud University Medical Center, Nijmegen, The Netherlands.

Advances in Microbial Physiology
|May 3, 2016
PubMed
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Proteomics reveals bacterial energy harvesting via electron transport phosphorylation. Advanced techniques offer untapped potential for studying microbial respiration and bioenergetics.

Keywords:
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Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
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Area of Science:

  • Microbiology
  • Biochemistry
  • Proteomics

Background:

  • Electron transport phosphorylation is key for prokaryotic energy production.
  • Microbial respiratory systems exhibit significant diversity and versatility.
  • Regulation of respiratory enzymes occurs at translational and posttranslational levels.

Purpose of the Study:

  • To provide an overview of proteomics methods for studying bacterial electron transport chains.
  • To explore the regulation of these chains from organism to protein structure levels.
  • To highlight underexploited opportunities in applying advanced proteomics to bacterial bioenergetics.

Main Methods:

  • Literature survey of proteomics techniques applied to bacterial respiration.
  • Analysis of regulatory mechanisms at molecular levels.
  • Comparison with proteomics applications in mitochondrial research.

Main Results:

  • Proteomics has significantly advanced understanding of bacterial respiratory mechanisms.
  • Combinations of proteomics with genetic and biochemical methods are highly effective.
  • Many advanced proteomics opportunities in bacterial bioenergetics remain underexploited.

Conclusions:

  • Proteomics is a powerful tool for elucidating bacterial electron transport and its regulation.
  • Further application of advanced proteomics can drive new discoveries in microbial bioenergetics.
  • Lessons from mitochondrial research can guide future studies in prokaryotes.