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Related Concept Videos

Proteomics01:33

Proteomics

10.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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The Proteasome02:18

The Proteasome

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Related Experiment Video

Updated: Mar 21, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Proteomics Moves into the Fast Lane.

Nicholas M Riley1, Alexander S Hebert2, Joshua J Coon3

  • 1Genome Center of Wisconsin, University of Wisconsin, Madison, WI 53706, USA; Department of Chemistry, University of Wisconsin, Madison, WI 53706, USA.

Cell Systems
|May 3, 2016
PubMed
Summary
This summary is machine-generated.

State-of-the-art mass spectrometry enables rapid and deep proteome characterization. These advancements offer powerful tools for comprehensive biological analysis.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Proteomics is crucial for understanding cellular functions.
  • Deep proteome characterization remains a challenge.

Purpose of the Study:

  • To highlight the capabilities of advanced mass spectrometry in proteomics.
  • To showcase rapid and in-depth proteome analysis.

Main Methods:

  • Utilizing state-of-the-art mass spectrometry techniques.
  • Applying advanced proteomics methodologies.

Main Results:

  • Demonstrated potential for rapid proteome characterization.
  • Achieved deep and comprehensive analysis of proteomes.

Conclusions:

  • Mass spectrometry-based proteomics offers significant advancements.
  • These methods facilitate efficient and thorough proteomic studies.