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Expression of CGRP in embryonic mouse masseter muscle
Yuri Azuma1, Yoko Miwa1, Iwao Sato1
1Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, Japan.
Summary
Calcitonin gene-related peptide (CGRP) influences mouse masseter muscle development, particularly embryonic myosin heavy chain and vascular markers during embryonic stages. Its expression is highest in early development, suggesting a key role in muscle and vascular formation.
Area of Science:
- Developmental Biology
- Molecular Biology
- Muscle Physiology
Background:
- Calcitonin gene-related peptide (CGRP) is known to mediate inflammation and influence vascular supply.
- CGRP's role in myoblast regulation and neuromuscular junctions is established, but its function during masseter muscle development is unclear.
Purpose of the Study:
- To investigate the expression and localization of CGRP during mouse masseter muscle (MM) development.
- To analyze the relationship between CGRP and key developmental markers including vascular genesis and myosin heavy chains.
Main Methods:
- Real-time RT-PCR was used to quantify mRNA levels of CGRP, vascular markers (VEGF-A, CD31, LYVE-1), and myosin heavy chains (MyHCs) at various developmental stages (E12.5-P5).
- In situ hybridization was employed to determine the spatial expression and localization of CGRP in the developing mouse MM.
Main Results:
- CGRP mRNA and protein were detected in the developing mouse MM from embryonic stages (E12.5-E17.5) but were not found after birth.
- CGRP expression levels correlated positively with embryonic MyHC and vascular markers (CD31, LYVE-1), peaking at embryonic day 17.5.
- Principal Component Analysis (PCA) and cluster analysis revealed distinct developmental patterns and a significant relationship between CGRP and embryonic MyHC.
Conclusions:
- CGRP plays a significant role during embryonic mouse masseter muscle development.
- The findings suggest CGRP influences embryonic MyHC expression and angiogenesis during MM development.
