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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 21, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Multicolor multiphoton in vivo imaging flow cytometry.

Lingjie Kong, Jianyong Tang, Meng Cui

|May 4, 2016

Summary

This summary is machine-generated.

This study introduces advanced in vivo imaging flow cytometry for non-invasive cell analysis. The new method reveals cell morphology and interactions, aiding disease diagnosis and therapeutic monitoring.

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Last Updated: Mar 21, 2026

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In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

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Area of Science:

Biomedical Engineering
Cellular Biology
Medical Imaging

Background:

In vivo flow cytometry enables non-invasive study of circulating cells in disease and therapy response.
Current methods lack detailed morphological information and fail to show intercellular interactions.

Purpose of the Study:

To develop and apply a multi-color, multiphoton in vivo imaging flow cytometry technique.
To enhance the study of circulating cell biology, disease progression, and therapeutic responses.

Main Methods:

Developed a multi-color, multiphoton in vivo imaging flow cytometry system.
Applied the system to image cellular dynamics in mouse bone marrow through the intact skull.
Utilized in situ deformability cytometry to distinguish cellular clusters and monitor cell trafficking.

Main Results:

Achieved high spatiotemporal resolution imaging of circulating cells within vasculature.
Successfully imaged cellular dynamics in bone marrow through intact mouse skull.
Distinguished cellular clusters and monitored multiple cell types based on morphology and fluorescence.

Conclusions:

The developed imaging flow cytometry offers unprecedented morphological and interaction insights.
This technique holds potential for early disease diagnosis and improved understanding of cellular dynamics.
Enables simultaneous monitoring of diverse cell populations in vivo.