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Related Experiment Videos

Mesenchymal-epithelial interactions between normal and transformed human bladder cells.

T R Pritchett1, J K Wang, P A Jones

  • 1Urological Cancer Research Laboratory, University of Southern California, Los Angeles 90033.

Cancer Research
|May 15, 1989
PubMed
Summary
This summary is machine-generated.

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Normal bladder cells and papillary carcinoma cells do not interact with fibroblasts. Invasive bladder carcinoma cells, however, infiltrate and grow with fibroblasts, suggesting a model for studying malignant progression.

Area of Science:

  • Urothelial biology
  • Cancer cell biology
  • Biomedical research

Background:

  • Understanding the tumor microenvironment is crucial for bladder cancer research.
  • Interactions between cancer cells and stromal cells influence tumor progression.
  • Investigating these interactions can reveal mechanisms of bladder cancer malignancy.

Purpose of the Study:

  • To investigate the differential interactions of normal urothelial cells, papillary bladder carcinoma cells, and invasive bladder carcinoma cells with bladder stromal cells.
  • To establish a model system for identifying factors involved in bladder cancer malignant progression.

Main Methods:

  • Culturing normal human urothelial cells, bladder carcinoma cell lines (papillary and invasive), and normal bladder stromal cells.
  • Utilizing mixed cultures to observe cell-cell interactions and growth responses.

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  • Employing a serum-free, fully defined medium for cell culture.

    • Main Results:

    • Normal urothelial cells showed no response to fibroblast growth factors and did not increase growth with fibroblast layers.
    • Papillary bladder carcinoma cells exhibited poor association with fibroblast layers and minimal growth stimulation.
    • Invasive bladder carcinoma cells (EJ line) readily infiltrated fibroblast layers and demonstrated significant growth stimulation by stromal cells.

    Conclusions:

    • The differential response of bladder cancer cells to stromal cells highlights the importance of the tumor microenvironment.
    • The EJ carcinoma cell line's interaction with fibroblasts provides a valuable model for studying invasive bladder cancer.
    • This model system can aid in identifying key molecular elements driving malignant progression in human bladder carcinoma.