Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activating p53<sup>Y220C</sup> with a mutant-specific small molecule.

Nature communications·2026
Same author

Correction: Discovery and optimization of tau targeted protein degraders enabled by patient induced pluripotent stem cells-derived neuronal models of tauopathy.

Frontiers in cellular neuroscience·2026
Same author

The E3-ome gene-centric compendium reveals the human E3 ligase landscape.

Cell·2026
Same author

Can ferric-oxyl excited states explain elongated iron-oxygen bonds in heme peroxidase catalytic intermediates?

Nature communications·2026
Same author

Crystallographic fragment screening supports tool compound discovery and reveals conformational flexibility in human deoxyhypusine synthase.

Communications chemistry·2026
Same author

Discovery of Histone Deacetylase 8-Specific Proteolysis-Targeting Chimeras with Anticancer Activity against Hematological Malignancies.

Journal of medicinal chemistry·2026

Related Experiment Video

Updated: Mar 21, 2026

Protein Crystallization for X-ray Crystallography
09:27

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

65.7K

A generic protocol for protein crystal dehydration using the HC1b humidity controller.

Carina M C Lobley1, James Sandy1, Juan Sanchez-Weatherby1

  • 1Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, England.

Acta Crystallographica. Section D, Structural Biology
|May 4, 2016
PubMed
Summary
This summary is machine-generated.

Crystal dehydration impacts X-ray diffraction data quality. This study developed a precise method to control humidity, revealing dehydration-induced structural changes in glucose isomerase crystals, including a space group shift.

Keywords:
cryoprotectioncrystal dehydrationgeneric protocolhumidity controlmacromolecular crystallizationroom temperature

More Related Videos

Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
11:14

Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins

Published on: January 6, 2017

8.5K
High-Throughput Protein Crystallization via Microdialysis
06:18

High-Throughput Protein Crystallization via Microdialysis

Published on: March 3, 2023

3.1K

Related Experiment Videos

Last Updated: Mar 21, 2026

Protein Crystallization for X-ray Crystallography
09:27

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

65.7K
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
11:14

Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins

Published on: January 6, 2017

8.5K
High-Throughput Protein Crystallization via Microdialysis
06:18

High-Throughput Protein Crystallization via Microdialysis

Published on: March 3, 2023

3.1K

Area of Science:

  • Crystallography
  • Structural Biology
  • Biophysics

Background:

  • Crystal dehydration can alter lattice parameters, affecting X-ray diffraction data quality (resolution, mosaicity).
  • Controlling the hydration environment around crystals is crucial for reproducible structural studies.
  • Previous methods for altering crystal hydration vary in precision and complexity.

Purpose of the Study:

  • To systematically investigate the impact of controlled dehydration on crystal structure and X-ray diffraction data.
  • To introduce a high-precision method for manipulating crystal hydration levels.
  • To assess the structural consequences of dehydration across diverse crystal systems.

Main Methods:

  • Utilized a high-precision humidifier/dehumidifier to generate controlled relative humidity airstreams.
  • Developed and applied a protocol for systematic dehydration experiments on nine different crystal systems.
  • Performed X-ray diffraction analysis to evaluate changes in crystal quality and structure.

Main Results:

  • Demonstrated that controlled dehydration significantly impacts crystal lattice, mosaicity, and resolution.
  • Observed a dehydration-induced phase transition in glucose isomerase, changing its space group from I222 to P21212.
  • Detailed the structural behavior of glucose isomerase during dehydration through extended studies.

Conclusions:

  • Controlled dehydration is a viable method to probe crystal structural dynamics and optimize diffraction data.
  • The observed space group change in glucose isomerase highlights the sensitivity of crystal structures to hydration levels.
  • The developed protocol offers a precise and accessible approach for studying dehydration effects in crystallography.