Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

5.1K
A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
5.1K
Glassware Calibration01:11

Glassware Calibration

1.9K
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
1.9K
Instrument Calibration01:12

Instrument Calibration

1.1K
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
1.1K
Focusing of Light in the Eye01:16

Focusing of Light in the Eye

7.2K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
7.2K
Calibration Curves: Correlation Coefficient01:10

Calibration Curves: Correlation Coefficient

5.5K
In a linear calibration curve, there is a value called the calibration coefficient, denoted by 'r,' which measures the strength and the direction of association between two variables. The correlation coefficient value ranges from −1 to +1. A value of +1 indicates a perfect positive linear correlation, −1 denotes a perfect negative correlation, and 0 implies no correlation between the two variables. A positive correlation value establishes that as one variable increases, the...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyclophilin A (CyPA) induces chemotaxis independent of its peptidylprolyl cis-trans isomerase activity: direct binding between CyPA and the ectodomain of CD147.

The Journal of biological chemistry·2011
Same author

Characterization of dissolved organic matter in urban sewage using excitation emission matrix fluorescence spectroscopy and parallel factor analysis.

Journal of environmental sciences (China)·2011
Same author

Actin filament associated protein mediates c-Src related SRE/AP-1 transcriptional activation.

FEBS letters·2011
Same author

Sesquiterpenes from Vladimiria souliei and their inhibitory effects on NO production.

Fitoterapia·2011
Same author

Chronic kidney disease is prevalent in Chinese patients admitted with verified cerebrovascular lesions and predicts short-term prognosis.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2011
Same author

Cytoprotective effects of albumin, nitrosated or reduced, in cultured rat pulmonary vascular cells.

American journal of physiology. Lung cellular and molecular physiology·2011

Related Experiment Video

Updated: Mar 21, 2026

Medical-grade Sterilizable Target for Fluid-immersed Fetoscope Optical Distortion Calibration
07:03

Medical-grade Sterilizable Target for Fluid-immersed Fetoscope Optical Distortion Calibration

Published on: February 23, 2017

8.1K

Method for out-of-focus camera calibration.

Tyler Bell, Jing Xu, Song Zhang

    Applied Optics
    |May 4, 2016
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel method for accurate camera calibration, even when the camera is significantly out of focus. It utilizes digital displays to generate fringe patterns, enabling precise feature point recovery despite substantial blurring.

    More Related Videos

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    11.3K
    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    14.0K

    Related Experiment Videos

    Last Updated: Mar 21, 2026

    Medical-grade Sterilizable Target for Fluid-immersed Fetoscope Optical Distortion Calibration
    07:03

    Medical-grade Sterilizable Target for Fluid-immersed Fetoscope Optical Distortion Calibration

    Published on: February 23, 2017

    8.1K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    11.3K
    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    14.0K

    Area of Science:

    • Computer Vision
    • Optical Engineering
    • Metrology

    Background:

    • Traditional camera calibration techniques require near-perfect focus, limiting their applicability in real-world scenarios with defocused imaging.
    • Substantial defocusing blurs feature points, rendering standard calibration methods ineffective and leading to inaccurate results.

    Purpose of the Study:

    • To develop a robust camera calibration method that functions accurately even with significant image defocus.
    • To overcome the limitations of existing calibration techniques that fail under defocused conditions.

    Main Methods:

    • Employing a digital display (e.g., liquid crystal display monitor) to generate structured fringe patterns.
    • Encoding feature points within the carrier phase of these fringe patterns for robust phase recovery.
    • Utilizing phase information to accurately locate feature points despite substantial blurring caused by defocus.

    Main Results:

    • Demonstrated accurate camera calibration irrespective of the degree of defocus.
    • Achieved a minimal focal length difference of approximately 0.2% between focused and substantially defocused states.
    • Showcased the resilience of the proposed method to image blur.

    Conclusions:

    • The proposed method enables accurate camera calibration for defocused systems, expanding the utility of calibration techniques.
    • This approach offers a practical solution for applications where maintaining perfect focus is challenging.
    • The findings suggest a significant advancement in optical metrology and machine vision systems operating under non-ideal focus conditions.