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Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health
Published on: October 9, 2017
A PLAUSIBLE ROLE FOR POLLEN-RESIDING MOLDS IN AGRICULTURAL PURPOSES
Abstract:
Pollen microbial content of 15 samples was investigated. Pollen was collected by honeybees. Total aerobic mesophilic count ranged from 3.00 to 5.48 Log CFU/g. Total mold and yeast count ranged from 2.3 to 6.99 Log CFU/g. Selected strains of isolated molds from pollen samples were characterized by conventional methods. Potent phytopathogenic and food spoilage species such as Penicillium sp., Alternaria alternata, Alternaria sp., Cladosporium werneckii, Mucor hiemalis, Rhizomucor pusillus, Aspergillus flavus, Aspergillus niger, Drechslera tritici-repentis, Verticillium albo-atrum, and Aspergillus alliaceus were recovered. Other fungal species with valuable biotechnological and plant diseases control purposes were isolated. They were characterized as Geotrichum candidum, Monilia sitophilia, and Sepedonium chrysospermum. Animal pathogenic molds were also isolated. Bee pollen may be considered as a source for a highly diverse fungal flora with different applications.
Insights
Bee pollen harbors a diverse fungal community, including potentially harmful and beneficial species. This study identified various molds, highlighting bee pollen
Area of Science:
- Microbiology
- Mycology
- Food Science
Background:
- Bee pollen is a nutrient-rich substance collected by honeybees.
- Pollen can harbor various microorganisms, including fungi.
- Understanding the fungal diversity in bee pollen is crucial for safety and potential applications.
Purpose of the Study:
- To investigate the microbial content of bee pollen samples.
- To identify and characterize fungal species present in bee pollen.
- To assess the potential risks and benefits associated with the fungal flora in bee pollen.
Main Methods:
- Collected 15 bee pollen samples.
- Quantified total aerobic mesophilic bacteria, mold, and yeast counts.
- Isolated and characterized fungal strains using conventional microbiological methods.
Main Results:
- Total aerobic mesophilic counts ranged from 3.00 to 5.48 Log CFU/g.
- Total mold and yeast counts ranged from 2.3 to 6.99 Log CFU/g.
- Identified phytopathogenic (e.g., Penicillium sp., Aspergillus flavus), food spoilage, animal pathogenic, and biotechnologically valuable fungi (e.g., Geotrichum candidum).
Conclusions:
- Bee pollen contains a highly diverse fungal flora.
- The identified fungi include species with pathogenic, spoilage, and beneficial properties.
- Bee pollen represents a potential source for fungal microorganisms with various applications.
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