[Establishing a 29 Y-STR Loci Multiplex PCR System].

Area of Science:

• Forensic genetics
• Molecular biology

Background:

• Y-chromosome Short Tandem Repeats (Y-STRs) are valuable genetic markers for male lineage identification.
• Established Y-STR multiplex systems are crucial for forensic casework and population genetics.

Purpose of the Study:

• To develop and validate a novel multiplex PCR system targeting 29 Y-STR loci.
• To assess the forensic application value of this 29 Y-STR system.

Main Methods:

• A five-color fluorescence-labeled multiplex PCR system was designed for 29 Y-STR loci.
• Capillary electrophoresis was used for amplification and analysis.
• The system's performance was validated using genetic data from 2,000 unrelated males in the Shandong Han population.

Main Results:

• The system achieved stable and accurate typing with high sensitivity (0.05 ng).
• 1,981 distinct haplotypes were identified among 2,000 individuals, demonstrating significant genetic diversity.
• The system is suitable for various routine biological samples.

Conclusions:

• The developed 29 Y-STR loci multiplex PCR system is applicable for forensic casework.
• This system can aid in the establishment of Y-STR databases.
• It holds significant value for forensic science practices and research.