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Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes
Mélanie Durand1,2, Elodie Segura3,4
1Institut Curie, Centre de recherche, 26 rue d'Ulm, 75248, Paris Cedex 05, France.
Methods in Molecular Biology (Clifton, N.J.)
|May 5, 2016
Abstract:
Dendritic cells (DCs) are a rare population of antigen-presenting cells that initiate immune responses in secondary lymphoid organs. In order to better understand the properties of DC in humans, it is essential to analyze DC subsets directly purified from tissues. Here, we describe a protocol allowing the purification of DC subsets from human tonsils and human lymph nodes.
