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Author Spotlight: Optimized Protocol for Detecting Antigen-Specific T Cells in Mouse Lungs Using Tetramers
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Protocols for the Identification and Isolation of Antigen-Presenting Cells in Human and Mouse Tissues
Naomi McGovern1, Andreas Schlitzer1, Baptiste Janela1
1Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, IMMUNOS Building #3-4, Biopolis, Singapore, 138648, Singapore.
Methods in Molecular Biology (Clifton, N.J.)
|May 5, 2016
Abstract:
The efficient processing of both mouse and human tissues is a valuable technique for characterizing tissue-associated immune cells. Here, we describe the techniques used and optimised within our laboratory for the enrichment and identification of antigen-presenting cells across a number of mouse and human tissues.

