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Two Novel Relative Double-Stranded RNA Mycoviruses Infecting Fusarium poae Strain SX63

Luan Wang1, Jingze Zhang2, Hailong Zhang3

  • 1State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. wluan@iccas.ac.cn.

International Journal of Molecular Sciences
|May 5, 2016
PubMed

Insights

Two new mycoviruses, Fusarium poae dsRNA virus 2 (FpV2) and FpV3, were identified in a plant pathogen. Their genomic and phylogenetic analyses support creating a new virus family, Fusagraviridae.

Area of Science:

  • Virology
  • Mycology
  • Molecular Biology

Background:

  • Plant pathogenic fungi like Fusarium poae harbor diverse viruses.
  • Double-stranded RNA (dsRNA) mycoviruses represent a significant, yet often uncharacterized, component of fungal viromes.

Purpose of the Study:

  • To isolate and molecularly characterize novel dsRNA mycoviruses from Fusarium poae.
  • To determine the genomic structure and phylogenetic relationships of these novel viruses.
  • To propose a new taxonomic classification for these dsRNA mycoviruses.

Main Methods:

  • Isolation of dsRNA mycoviruses from Fusarium poae strain SX63.
  • Genome sequencing and analysis of open reading frames (ORFs) and conserved domains.
  • Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) sequences.
  • Comparative analysis of genome organization.

Main Results:

  • Discovery and characterization of two novel dsRNA mycoviruses, FpV2 (9518 bp) and FpV3 (9419 bp).
  • Both viruses possess two discontinuous ORFs encoding a hypothetical protein (P1) and an RdRp, with conserved Phytoreo_S7 and RdRp_4 domains.
  • Evidence of programmed -1 ribosomal frameshifting (-1 PRF) mediated by a shifty heptamer (GGAAAAC) in ORF1.
  • Phylogenetic analysis places FpV2 and FpV3 within an unassigned dsRNA mycovirus group.

Conclusions:

  • FpV2 and FpV3 represent distinct species within a proposed new taxonomic family, Fusagraviridae.
  • The findings expand our understanding of dsRNA mycovirus diversity and evolution.
  • This classification provides a framework for future studies on related mycoviruses.
Keywords:
FpV2FpV3FusagraviridaeFusarium poaedsRNA virusnovel mycovirus

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