Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Full length TECPR1 displays 'cis' Dysferlin domain architecture.

bioRxiv : the preprint server for biology·2026

Same author

Mechanistic insights into CAM-induced disruption of HBV capsids revealed by all-atom MD simulations.

PLoS pathogens·2026

Same author

Structure and signaling mechanism of <i>Helicobacter pylori</i> transducer-like protein D.

bioRxiv : the preprint server for biology·2026

Same author

Transient protein structure guides surface diffusion pathways for electron transport in membrane supercomplexes.

Nature communications·2026

Same author

Novel adenovirus vaccine vectors lacking thrombosis-associated interactions with platelet factor 4.

iScience·2026

Same author

Prebiotically Plausible Peptides can Self-assemble into β-rich Nanostructures.

bioRxiv : the preprint server for biology·2025

Same journal

A Tubules-First Model for the Origin of Eukaryotic Membrane Traffic.

Annual review of biophysics·2026

Same journal

Seeking Biology's Physics Stories: Simplify, Simplify.

Annual review of biophysics·2026

Same journal

Pattern Formation Beyond Turing: Physical Principles of Mass-Conserving Reaction-Diffusion Systems.

Annual review of biophysics·2026

Same journal

Rigidity and Mechanical Response in Biological Structures.

Annual review of biophysics·2026

Same journal

Systems Biology of Aging, Metabolism, and Mitochondria.

Annual review of biophysics·2026

Same journal

Ligand Binding Dynamics of Ion Channels and GPCRs Using Single-Molecule Fluorescence.

Annual review of biophysics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Computational Methodologies for Real-Space Structural Refinement of Large Macromolecular Complexes.

Boon Chong Goh^1,2, Jodi A Hadden^1,3, Rafael C Bernardi^1,3

¹Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.

Annual Review of Biophysics

|May 6, 2016

Summary

This summary is machine-generated.

Computers enhance biomolecular structure determination by integrating diverse experimental data into atomistic models. Computational methods are crucial for refining structures of large complexes like ribosomes and viruses.

Keywords:

cryo-EM flexible fitting hybrid methods integrative modeling molecular dynamics simulation

More Related Videos

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

Related Experiment Videos

Last Updated: Mar 21, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

Area of Science:

Structural Biology
Computational Biology
Biophysics

Background:

Advanced experimental techniques provide high-resolution structural data for biomolecular systems.
Integrating data from multiple sources (e.g., X-ray crystallography, cryo-EM) is challenging for large complexes.
Computational tools are increasingly vital for analyzing and interpreting complex structural data.

Purpose of the Study:

To review computational strategies for real-space structural refinement of large biomolecular systems.
To illustrate the application of these methods using examples of macromolecular complexes.
To discuss the current and future role of computational approaches in structural biology.

Main Methods:

Computational hybrid methods integrating multimodal experimental data.
Real-space structural refinement techniques.
Application examples: ribosome, virus capsids, chemosensory array, photosynthetic chromatophore.

Main Results:

Computational methods successfully integrate diverse data into consistent, atomistic structures.
Demonstrated utility in refining structures of large, complex biological assemblies.
Highlighting the power of computational approaches in modern structural biology.

Conclusions:

Computers and computational methods are revolutionizing biomolecular structure determination.
Hybrid computational approaches are essential for resolving complex macromolecular structures.
Future challenges and opportunities exist in large-scale structural refinement.