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Immunological detection of penicillin-binding protein 2' in clinical isolates of methicillin-resistant Staphylococcus
D M O'Hara1, C R Harrington, P E Reynolds
1Department of Biochemistry, University of Cambridge, U.K.
FEMS Microbiology Letters
|January 1, 1989
Abstract:
The additional penicillin-binding protein (PBP 2') that is important in determining intrinsic resistance in methicillin-resistant strains of Staphylococcus aureus (MRSA) has been detected immunologically in strains from a variety of world-wide locations. This additional protein has also been definitively identified both immunologically and as a PBP in methicillin-resistant strains of S. epidermidis (MRSE). The assay described is rapid, specific and sensitive and has been used to detect PBP 2' in S. haemolyticus but not in beta-lactam resistant Streptococci.