Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels.

npj biological physics and mechanics·2026

Same author

Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics.

Nucleus (Austin, Tex.)·2026

Same author

Deep Invaginations of Nuclear Envelope Coordinate Spatial Organization of Chromatin in Epithelium.

bioRxiv : the preprint server for biology·2026

Same author

Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics.

bioRxiv : the preprint server for biology·2026

Same author

A-type lamins anchor emerin at the inner nuclear membrane via two independent binding sites.

The Journal of biological chemistry·2026

Same author

A subnanolitre tetherless optoelectronic microsystem for chronic neural recording in awake mice.

Nature electronics·2025

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Published on: July 5, 2022

Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling.

Gregory Fedorchak¹, Jan Lammerding²

¹School of Biomedical Engineering, Weill Institute for Cell and Molecular Biology, Cornell University, 235 Weill Hall, Ithaca, NY, 14853-7202, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 6, 2016

Summary

This summary is machine-generated.

This study developed a new assay to measure how forces move within cells, revealing insights into how the nucleus and cytoskeleton interact and how this impacts cell function.

Keywords:

Cell mechanics Cell signaling Force transmission LINC complex Lamins Mechanotransduction Nesprin SUN proteins

More Related Videos

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes

Published on: January 12, 2024

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

Related Experiment Videos

Last Updated: Mar 21, 2026

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

Published on: July 5, 2022

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes

Author Spotlight: Elucidating the Dynamics of Mechano-Transduction and Nuclear Agitation in Mouse Oocytes

Published on: January 12, 2024

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

Area of Science:

Cell biology
Biophysics
Mechanobiology

Background:

The nucleus and cytoskeleton are critical cellular components.
Understanding their mechanical interactions is key to cell function and disease.

Purpose of the Study:

To develop and optimize a novel assay for evaluating intracellular force transmission.
To quantitatively assess the coupling between the nucleus and cytoskeleton.

Main Methods:

Utilized a single cell-based assay with micromanipulation of adherent cells using a glass microneedle.
Employed a custom-programmable computer script for controlled needle manipulation.
Applied normalized cross-correlation to time-lapse image sequences to measure regional displacements.
Analyzed nuclear elongation, centroid translocation, and shape changes.

Main Results:

Successfully optimized a micromanipulation assay for quantifying nucleo-cytoskeletal force transmission.
Demonstrated the ability to measure regional displacements in the nucleus and cytoskeleton.
Provided quantitative data on nucleo-cytoskeletal coupling.

Conclusions:

The developed assay enables quantitative assessment of nucleo-cytoskeletal coupling.
Offers improved understanding of intracellular force propagation.
Facilitates research into the role of nuclear envelope proteins in force transmission and disease states.