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Updated: Mar 21, 2026

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

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Rev-RRE Functional Activity Differs Substantially Among Primary HIV-1 Isolates.

Patrick E Jackson¹, Denis M Tebit¹, David Rekosh¹

¹Department of Microbiology, Immunology, and Cancer Biology, Myles H. Thaler Center for AIDS and Human Retrovirus Research, University of Virginia , Charlottesville, Virginia.

AIDS Research and Human Retroviruses

|May 6, 2016

Summary

HIV-1

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Last Updated: Mar 21, 2026

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Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Published on: April 9, 2014

Area of Science:

Virology
Molecular Biology
Genetics

Background:

HIV-1 replication depends on exporting viral RNA, a normally restricted process.
The viral Rev protein and its Rev response element (RRE) facilitate this export.
Understanding sequence variations in Rev-RRE interactions is crucial.

Purpose of the Study:

To investigate how sequence differences in Rev-RRE pairs affect functional activity.
To explore the implications of these variations in HIV-1 pathogenesis and lentiviral vector development.

Main Methods:

Utilized a lentiviral vector assay to measure Rev-RRE pair activity.
Quantified functional activity differences across naturally occurring viral isolates.

Main Results:

Significant functional variation (up to 24-fold) observed in Rev-RRE pairs from different HIV-1 isolates.
Activity differences correlated with Rev protein, not RRE sequence, and were independent of Rev protein levels.
Identified substantial variation driven by Rev sequence differences.

Conclusions:

HIV-1 Rev protein sequence variations significantly impact Rev-RRE functional activity.
This variation may influence viral adaptation, immune evasion, and latency establishment.
Findings could enhance lentiviral vector production efficiency.