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Microbes in Beverage Production01:25

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Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
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Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
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Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
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Flor Yeast: New Perspectives Beyond Wine Aging.

Jean-Luc Legras1, Jaime Moreno-Garcia2, Severino Zara3

  • 1SPO, Institut National de la Recherche Agronomique - SupAgro, Université de Montpellier Montpellier, France.

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|May 6, 2016
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Summary

Flor yeast, crucial for Sherry wine aging, forms biofilms through unique genetic traits. Omics tools reveal how this lifestyle reshapes yeast proteins, offering new ways to control biofilm formation.

Keywords:
-omic toolsbiocapsulesbiofilmbiofilm managementflor yeastimmobilizationwine

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Area of Science:

  • Enology
  • Microbiology
  • Biochemistry

Background:

  • White wine production prioritizes aroma preservation and limits oxygen exposure.
  • Sherry wine aging is an aerobic process reliant on flor yeast (Saccharomyces cerevisiae).
  • Flor yeast form biofilms (flor or velum) on wine surfaces under nutrient limitation.

Purpose of the Study:

  • To review current understanding of flor yeast biology using omics tools.
  • To explore the genetic and metabolic adaptations of flor yeast.
  • To discuss factors influencing biofilm formation and potential applications.

Main Methods:

  • Comparative genomics, proteomics, and metabolomics analyses.
  • Investigation of flor yeast versus non-flor wine yeast strains.
  • Review of existing literature on biofilm formation factors and strategies.

Main Results:

  • Omics data highlight unique features of flor yeast differentiating them from other wine yeasts.
  • Significant proteome remodeling occurs in flor yeast due to their biofilm lifestyle.
  • Flor yeast exhibit specific genetic and metabolic pathways enabling biofilm formation.

Conclusions:

  • Flor yeast possess distinct characteristics enabling their role in aerobic wine aging.
  • Understanding flor yeast proteomics and genomics provides insights into biofilm development.
  • Potential exists for manipulating biofilm formation for winemaking applications.