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Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device.

Jinghui Luo1,2, Katherine A Muratore1,2, Edgar A Arriaga1,2

  • 1School of Molecular Sciences and ⊥Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.

Analytical Chemistry
|May 6, 2016
PubMed
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This study introduces a novel microfluidic method for separating micron and submicron particles. The technique utilizes deterministic absolute negative mobility (dANM) to achieve highly efficient particle separation, even for biological samples like mitochondria.

Area of Science:

  • Microfluidics and Nanotechnology
  • Biophysics and Cell Biology
  • Materials Science

Background:

  • Efficient separation of micron and submicron particles is crucial for nanotechnology and biological applications.
  • Existing separation methods often lack the required efficiency and selectivity for complex biological samples.

Purpose of the Study:

  • To demonstrate a novel, nonintuitive separation mechanism for micron and submicron colloidal particles and organelles.
  • To achieve size-selective separation using deterministic absolute negative mobility (dANM) in a microfluidic device.

Main Methods:

  • Utilized a microfluidic device with a nonlinear post array.
  • Applied periodic electrokinetic and dielectrophoretic forces to induce particle transport.
  • Employed numerical modeling for characterization and optimization, followed by experimental implementation.

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Last Updated: Mar 21, 2026

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Main Results:

  • Demonstrated deterministic absolute negative mobility (dANM) where larger particles migrate opposite to the applied force.
  • Achieved size selectivity, with smaller particles exhibiting normal migration.
  • Obtained migration speeds at least two orders of magnitude higher than previous ANM systems.
  • Successfully induced dANM for mouse liver mitochondria.

Conclusions:

  • The developed microfluidic system offers a highly efficient and size-selective separation mechanism.
  • Deterministic absolute negative mobility is a viable strategy for separating nanoparticles, organelles, and protein nanocrystals.
  • This technology has significant potential for future applications in biological and nanotechnological sample preparation and analysis.