Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method for protein labeling with 99mTc.

D Blok1, R I Feitsma, M N Wasser

  • 1Department of Diagnostic Radiology, University Hospital Leiden, The Netherlands.

International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Global plant trait relationships extend to the climatic extremes of the tundra biome.

Nature communications·2020
Same author

Traditional plant functional groups explain variation in economic but not size-related traits across the tundra biome.

Global ecology and biogeography : a journal of macroecology·2019
Same author

Integrating patient safety education into early medical education utilizing cadaver, sponges, and an inter-professional team.

BMC medical education·2018
Same author

Accuracy of MRI for treatment response assessment after taxane- and anthracycline-based neoadjuvant chemotherapy in HER2-negative breast cancer.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2014
Same author

Reply.

European journal of nuclear medicine·2014
Same author

Addition of zoledronic acid to neoadjuvant chemotherapy does not enhance tumor response in patients with HER2-negative stage II/III breast cancer: the NEOZOTAC trial (BOOG 2010-01).

Annals of oncology : official journal of the European Society for Medical Oncology·2014
Same journal

Photoreactive 111In-cyclodextrin inclusion complex: a new heterobifunctional reagent for antibody labeling.

International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology·1992
Same journal

(S)- and (R)-[11C]nicotine and the metabolite (R/S)-[11C]cotinine. Preparation, metabolite studies and in vivo distribution in the human brain using PET.

International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology·1992
Same journal

Ligands and tracers for PET studies of the 5-HT system--current status.

International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology·1992
Same journal

Evaluation of S-[11C]citalopram as a radioligand for in vivo labelling of 5-hydroxytryptamine uptake sites.

International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology·1992
Same journal

Attachment of 99mTc to lipid vesicles containing the lipophilic chelate dipalmitoylphosphatidylethanolamine-DTTA.

International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology·1992
Same journal

A bifunctional HBED-derivative for labeling of antibodies with 67Ga, 111In and 59Fe. Comparative biodistribution with 111In-DPTA and 131I-labeled antibodies in mice bearing antibody internalizing and non-internalizing tumors.

International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology·1992
See all related articles

This study presents a method for labeling proteins with technetium-99m (99mTc), ideal for diagnostic nuclear medicine. The technique ensures efficient labeling, stable attachment, and preserved biological function, demonstrated with an anti-fibrin antibody for thrombus imaging.

Area of Science:

  • Biochemistry
  • Radiochemistry
  • Immunology

Background:

  • Technetium-99m (99mTc) is the preferred radionuclide for diagnostic nuclear medicine due to its favorable imaging properties.
  • Radiolabeling of biomolecules, particularly monoclonal antibodies, is crucial for targeted diagnostic and therapeutic applications.
  • Ensuring the stability and retention of biological function post-labeling is a key challenge in developing radiopharmaceuticals.

Purpose of the Study:

  • To describe a novel method for labeling proteins with 99mTc.
  • To evaluate the efficiency and stability of the 99mTc radiolabeling process.
  • To demonstrate the retention of biological activity, such as immunoreactivity, in radiolabeled monoclonal antibodies.
  • To present an application of a 99mTc-labeled anti-fibrin monoclonal antibody for radioimmunoimaging of thrombi.

Related Experiment Videos

Main Methods:

  • Development and optimization of a 99mTc protein labeling protocol.
  • Assessment of labeling efficiency using appropriate radiochemical assays.
  • Evaluation of label attachment stability under physiological conditions.
  • Characterization of retained biological behavior, including immunoreactivity of labeled monoclonal antibodies.
  • In vivo studies using a 99mTc-labeled anti-fibrin monoclonal antibody for thrombus radioimmunoimaging.

Main Results:

  • The described method achieves high labeling efficiency for proteins with 99mTc.
  • The radiolabel attachment demonstrates excellent stability, resisting dissociation under relevant conditions.
  • Monoclonal antibodies labeled using this method retain their biological activity, specifically immunoreactivity.
  • The 99mTc-labeled anti-fibrin monoclonal antibody successfully enabled radioimmunoimaging of thrombi, demonstrating its potential clinical utility.

Conclusions:

  • A robust and efficient method for 99mTc radiolabeling of proteins has been established.
  • This technique preserves the critical biological functions of labeled biomolecules, such as antibodies.
  • The successful application in thrombus radioimmunoimaging highlights the potential of 99mTc-labeled anti-fibrin antibodies in diagnostic nuclear medicine.