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Plasma Lipidomics Investigation of Hemodialysis Effects by Using Liquid Chromatography-Mass Spectrometry

Lichao Wang1,2, Chunxiu Hu2, Shuxin Liu3

  • 1College of Chemical Engineering, Zhejiang University of Technology , Hangzhou 310014, China.

Journal of Proteome Research
|May 7, 2016
PubMed

Insights

Chronic kidney disease (CKD) patients undergoing hemodialysis (HD) exhibit significant alterations in lipid metabolism. Hemodialysis impacts levels of free fatty acids and other lipids, offering insights into uremia-related complications.

Area of Science:

  • Biochemistry
  • Metabolomics
  • Nephrology

Background:

  • Chronic kidney disease (CKD) is a global health issue, often progressing to uremia.
  • Hemodialysis (HD) is a primary treatment for uremia, but patients face complications like lipid disorders.

Purpose of the Study:

  • To investigate the effects of hemodialysis on lipid metabolism in uremic patients.
  • To utilize lipidomics to understand complex complications in HD patients.

Main Methods:

  • Employed ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) based lipidomics.
  • Analyzed 87 human plasma samples from pre-hemodialysis (pre-HD), post-hemodialysis (post-HD), and healthy control groups.

Main Results:

  • Significant changes in plasma lipid profiles were observed in HD patients compared to controls (p < 0.05).
  • Levels of free fatty acids (FFA), saturated FFA, eicosanoids, lyso-phosphatidylinositols, and lyso-phosphatidylethanolamines were elevated pre-HD but decreased post-HD.
  • Specific ratios like FFA 16:1/FFA 16:0 and FFA 18:1/FFA 18:0 also showed significant changes.

Conclusions:

  • UPLC-Q-TOF/MS lipidomics is a valuable tool for studying uremia-related lipid alterations.
  • Findings contribute to understanding the complex complications associated with hemodialysis therapy.

Keywords:
Chronic kidney diseasehemodialysislipidomicsliquid chromatography−flight mass spectrometryplasma lipids

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