Electrochemical non-enzyme sensor for detecting clenbuterol (CLB) based on MoS₂-Au-PEI-hemin layered nanocomposites

Clenbuterol (CLB), a kind of β₂-adrenergic agonists, could disrupt cardiovascular and central nervous system. In this work, a new electrochemical non-enzyme sensor for detecting Clenbuterol (CLB) was fabricated based on MoS₂-Au-PEI-hemin layered nanocomposites. The two-dimensional (2-D) MoS₂ nanosheets were first in-situ assembled with Au nanoparticles, and polyethylenimine (PEI), then hemin molecules were immobilized onto the MoS₂-Au-PEI film-modified glassy carbon electrode (GCE) via amide bond. Scanning electron microscopy (SEM) and Zeta potential measurements were employed to characterize the MoS₂-based nanomaterials. Cyclic voltammetry (CV) was used to investigate electrochemical activity of the immobilized hemin on the modified electrode. Upon the optimum conditions, the proposed electrochemical sensor showed an excellent response for CLB including a wide linear ranging from 10ng/mL to 2μg/mL and a detection limit (LOD) of 1.92ng/mL CLB (S/N=3) with favorable reproducibility and stability. Furthermore, this presented method could be feasible for determining CLB in the real pork samples.