Draft Genome Sequence of Vancomycin-Susceptible, Ampicillin-Intermediate Enterococcus faecium Strain D344RRF

Area of Science:

• Microbiology
• Genomics
• Infectious Diseases

Background:

• Enterococcus faecium is a significant cause of hospital-acquired infections.
• High antibiotic resistance rates in E. faecium contribute to a major health burden.
• The bacterium's ability to colonize the gastrointestinal tract facilitates its spread.

Purpose of the Study:

• To provide the draft genome sequence of a specific Enterococcus faecium laboratory strain.
• To characterize the antibiotic resistance profile of strain D344RRF.
• To highlight the utility of this strain in research on antibiotic resistance mechanisms.

Main Methods:

• Whole-genome sequencing of Enterococcus faecium strain D344RRF.
• Bioinformatic analysis of the generated genome data.
• Phenotypic characterization of antibiotic susceptibility.

Main Results:

• The draft genome sequence for vancomycin-susceptible, ampicillin-intermediate E. faecium strain D344RRF was obtained.
• Strain D344RRF displays resistance to rifampicin and fusidic acid.
• This characterized genome provides a resource for resistance studies.

Conclusions:

• The genome of E. faecium D344RRF is a valuable resource for investigating antibiotic resistance.
• This strain's specific resistance profile makes it suitable for studying resistance transfer mechanisms.
• Further research utilizing this genomic data can advance understanding of nosocomial pathogens.