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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Mar 21, 2026

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

Mass Cytometry: Single Cells, Many Features.

Matthew H Spitzer¹, Garry P Nolan²

¹Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA; Program in Immunology, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University, Stanford, CA 94305, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

|May 7, 2016

Summary

This summary is machine-generated.

Mass cytometry combines flow cytometry and mass spectrometry to measure over 40 cellular features simultaneously at single-cell resolution, advancing biological research capabilities.

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Last Updated: Mar 21, 2026

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

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Sample Preparation for Mass Cytometry Analysis

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Area of Science:

Biological research technology
Single-cell analysis
Cytometry

Background:

Flow cytometry enables measurement of multiple cellular features in single cells.
Traditional flow cytometry balances feature number and resolution.
Complex cellular systems require advanced analytical tools.

Purpose of the Study:

To review the current state of mass cytometry.
To provide an overview of mass cytometry instrumentation, capabilities, and data analysis.
To discuss future developments and applications of mass cytometry.

Main Methods:

Leveraging the precision of mass spectrometry with flow cytometry.
Development of mass cytometry instrumentation.
Application of mass spectrometry principles to cellular analysis.

Main Results:

Mass cytometry enables measurement of over 40 simultaneous cellular parameters.
Achieves single-cell resolution for complex biological samples.
Significantly augments the evaluation of complex cellular systems and processes.

Conclusions:

Mass cytometry represents a significant advancement in cytometry.
It enhances the ability to study intricate cellular functions.
Future developments promise broader applications in biological research.