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Updated: Mar 21, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
Published on: April 19, 2021
Efficient identification and analysis of chemical exchange in biomolecules by R1ρ relaxation dispersion with
Erik Walinda1, Daichi Morimoto1, Mayu Nishizawa1
1Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-Ku, Kyoto, 615-8510, Japan.
Unlabelled:
We introduce here a novel acquisition and processing methodology for cross-polarization based 1D rotating-frame relaxation dispersion NMR experiments. This easy-to-use protocol greatly facilitates the screening, acquisition, processing and model fitting of large on- and off-resonance R1ρ relaxation dispersion NMR datasets in an automated manner for the analysis of chemical exchange phenomena in biomolecules.
Availability And Implementation:
The Amaterasu package including the spreadsheet, Bruker pulse programs and analysis software is available at www.moleng.kyoto-u.ac.jp/∼moleng_01/amaterasu
Contact:
: sugase@moleng.kyoto-u.ac.jp.
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