Related Experiment Video
Updated: Mar 21, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
Differentiation of aged fibers by Raman spectroscopy and multivariate data analysis
Federica Bianchi1, Nicolò Riboni1, Valentina Trolla1
1Dipartimento di Chimica, Università degli Studi di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.
Abstract:
Raman spectroscopy followed by multivariate data analysis was used to analyze cotton fibers dyed using similar formulations and submitted to different aging conditions. Spectra were collected on a commercial instrument using a near-infrared laser with a 780nm light source. Discriminant analysis allowed to correctly classify the aged fibers 100% of the time. The prediction ability of the calculated model was estimated to be 100% by the "leave-one-out" cross-validation for 3 out of the 4 series under investigation. Finally, reliability of the developed approach for the discrimination of aged vs new fibers was confirmed by the analysis of commercial polyamide and polyester textiles submitted to the same aging process.
More Related Videos
07:37An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
Published on: January 9, 2020
07:51Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
Published on: June 10, 2017
Related Concept Videos
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
Classification of Skeletal Muscle Fibers
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...