Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

17.7K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
17.7K
Ligand Binding Sites02:40

Ligand Binding Sites

15.7K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
15.7K
Factors Affecting Dissolution: Drug pKa, Lipophilicity and GI pH01:21

Factors Affecting Dissolution: Drug pKa, Lipophilicity and GI pH

4.1K
Drug absorption within the gastrointestinal (GI) tract is a complex process influenced by several critical factors, including the site pH, the drug's dissociation constant (pKa), and the drug's lipophilicity. The GI tract exhibits a pH gradient, with an acidic environment in the stomach and a more alkaline environment in the small intestine. This pH variation directly affects the ionization state of drugs.
A drug's pKa and the pH of the gastrointestinal (GI) tract play crucial roles...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Following phospholipid transfer through the OmpF<sub>3</sub>-MlaA-MlaC lipid shuttle with native mass spectrometry.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Membrane Protein Complexity Revealed Through Native Mass Spectrometry.

Annual review of biochemistry·2025
Same author

Traversing the drug discovery landscape using native mass spectrometry.

Current opinion in structural biology·2025
Same author

Defining proteoform-specific interactions for drug targeting in a native cell signalling environment.

Nature chemistry·2025
Same author

Structural insights into the high basal activity and inverse agonism of the orphan receptor GPR6 implicated in Parkinson's disease.

Science signaling·2024
Same author

Coupling and Activation of the β1 Adrenergic Receptor - The Role of the Third Intracellular Loop.

Journal of the American Chemical Society·2024

Related Experiment Video

Updated: Mar 21, 2026

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs
09:45

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs

Published on: February 5, 2022

4.1K

A sliding selectivity scale for lipid binding to membrane proteins.

Michael Landreh1, Michael T Marty1, Joseph Gault1

  • 1Department of Chemistry, Physical & Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, Oxfordshire, OX1 3QZ, United Kingdom.

Current Opinion in Structural Biology
|May 8, 2016
PubMed
Summary

Biological membranes utilize proteins that interact with lipids on a sliding selectivity scale. This dynamic organization is crucial for cellular integrity and function, as revealed by mass spectrometry studies.

More Related Videos

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

628
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
04:45

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

8.0K

Related Experiment Videos

Last Updated: Mar 21, 2026

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs
09:45

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs

Published on: February 5, 2022

4.1K
A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

628
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
04:45

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Published on: February 10, 2022

8.0K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Biological membranes are essential barriers for cellular integrity.
  • Membrane proteins interact with lipids, ranging from non-selective to highly specific interactions.
  • Lipid-protein interactions influence protein structure and dynamics within the membrane.

Purpose of the Study:

  • To explore the biological implications of various lipid-binding scenarios with membrane proteins.
  • To propose a model of lipid binding on a sliding selectivity scale.
  • To understand the dynamic organization of biological membranes.

Main Methods:

  • Utilized mass spectrometry to monitor lipid-protein interactions.
  • Assessed the effects of lipids on membrane protein structure and dynamics.
  • Analyzed different lipid-binding modes: specific, preferential, and nonselective.

Main Results:

  • Demonstrated the ability to differentiate between specific lipid binding, preferential lipid interactions, and nonselective annular contacts.
  • Observed a spectrum of lipid selectivity in protein interactions.
  • Highlighted the dynamic nature of lipid-protein organization.

Conclusions:

  • Lipid binding to membrane proteins operates on a sliding selectivity scale.
  • Biological membranes facilitate dynamic protein and lipid organization.
  • Understanding these interactions is key to cellular integrity and function.