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Genome characterization of feline morbillivirus from Italy

Maurilia Marcacci1, Eliana De Luca1, Guendalina Zaccaria1

  • 1Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.

Journal of Virological Methods
|May 8, 2016
PubMed

Insights

Feline morbillivirus (FeMV) was identified in a sick cat

Area of Science:

  • Veterinary Virology
  • Molecular Biology
  • Genomics

Background:

  • Feline morbillivirus (FeMV) is an emerging viral pathogen affecting domestic cats.
  • Previous identification of FeMV was primarily through RT-PCR in urine samples.

Purpose of the Study:

  • To determine the whole genome sequence of the FeMV strain Piuma/2015.
  • To investigate the genetic diversity of FeMV in Europe.

Main Methods:

  • RNA extraction from infected feline urine.
  • Sequence-independent single primer amplification (SISPA).
  • Next-generation sequencing (NGS).

Main Results:

  • The complete genome sequence of the FeMV Piuma/2015 strain was successfully obtained.
  • FeMV strains found in Germany and Turkey exhibit genetic divergence from the Italian strain.
  • This indicates FeMV genetic heterogeneity within Europe.

Conclusions:

  • The study provides the full genome sequence for FeMV strain Piuma/2015.
  • Evidence suggests FeMV is genetically diverse across Europe, similar to findings in Asia.
  • Further research is needed to understand the implications of FeMV genetic variation.
Keywords:
CatFeline morbillivirusItalyNGSSISPAUrine

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