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Updated: Mar 21, 2026

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research
Published on: October 27, 2023
Variations in the technique for autologous limbal transplantation
B Mataix1, A Alcántara1, M Caro1
1Sección de Superficie Ocular y Córnea, Servicio de Oftalmología, Hospital Universitario Virgen Macarena, Sevilla, España.
Objective:
To present the results on the use of a single block limbal autograft, combined with amniotic membrane transplantation and sectoral sequential postoperative epitheliectomy of the conjunctiva in 2 patients with unilateral total limbal stem cell deficiency.
Conclusions:
A single block limbal autograft combined with amniotic membrane transplantation may be sufficient to restore a stable corneal surface, but sometimes sequential sectoral conjunctival epitheliectomy may be required to treat anomalous epithelial remnants.

