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Updated: Mar 21, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Oncogenic viruses: Lessons learned using next-generation sequencing technologies

Ronan Flippot1, Gabriel G Malouf1, Xiaoping Su2

  • 1University Hospital Pitié Salpêtrière, Department of Medical Oncology, University Pierre and Marie Curie, Paris, France.

European Journal of Cancer (Oxford, England : 1990)
|May 9, 2016
PubMed

Insights

Fifteen percent of cancers stem from oncogenic human viruses that integrate into host DNA. Understanding these viral integration mechanisms is key to developing better cancer prognoses and therapies.

Area of Science:

  • Oncology
  • Virology
  • Genomics

Background:

  • Approximately 15% of human cancers are linked to oncogenic viruses.
  • Four key viruses (hepatitis B virus, human papillomavirus, Merkel cell polyomavirus, human T-cell lymphotropic virus) integrate into the host genome.
  • Viral integration can lead to oncogenesis through epigenetic and genetic alterations.

Purpose of the Study:

  • To provide novel insights into the initiating events of cancer onset caused by integrative viruses.
  • To highlight the role of viral integration in cancer development.
  • To discuss potential clinical implications and future research directions.

Main Methods:

  • Leveraging high-throughput sequencing and advanced bioinformatic tools.
  • Analyzing large datasets to map genomic alterations following viral integration.
  • Investigating epigenetic and genetic modifications induced by viral integration.

Main Results:

  • Established the landscape of genomic alterations associated with viral integration.
  • Identified key events occurring post-integration that contribute to cancer onset.
  • Provided a foundation for predicting consequences of viral integration.

Conclusions:

  • Viral integration is a critical factor in a significant portion of virus-driven cancers.
  • Further research into integration mechanisms will improve cancer classification and treatment.
  • Advancements in sequencing and bioinformatics are crucial for understanding viral oncogenesis.
Keywords:
CancerIntegrationNGSNext-generation sequencingVirus

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