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Published on: July 16, 2018
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Microarray-based Gene Expression Profiling of Abdominal Aortic Aneurysm.
Summary
Gene expression in abdominal aortic aneurysms (AAA) differs from controls, with specific genes showing altered levels. These changes, related to inflammation and cell death, are linked to AAA pathogenesis and repair.
Area of Science:
- Molecular Biology
- Genomics
- Vascular Biology
Background:
- Abdominal aortic aneurysms (AAA) are characterized by complex molecular changes.
- Gene expression profiling offers potential for identifying prognostic biomarkers in AAA.
Purpose of the Study:
- To compare peripheral blood gene expression between individuals with AAA and controls.
- To identify differentially expressed genes and pathways involved in AAA pathogenesis.
Main Methods:
- Microarray analysis of peripheral blood transcriptome in 12 AAA patients and 12 controls.
- Validation of 11 differentially expressed genes using quantitative PCR in independent cohorts, including pre- and post-repair patients and tissue samples.
Main Results:
- Microarray identified 47 significantly differentially expressed genes in AAA, involved in apoptosis, proteolysis, and immune response.
- Validation confirmed downregulation of MSN, PSMB10, and STIM1 in AAA patients; PSMB10 showed consistent downregulation.
- Expression of 11 key genes was detected in aortic biopsies and smooth muscle cells.
Conclusions:
- Peripheral blood transcriptomic analysis reveals differential gene expression in AAA patients.
- Identified genes and pathways (proteolysis, inflammation, apoptosis) are implicated in AAA pathogenesis.
- Gene expression changes are modulated by aneurysm repair and present in aortic tissues.
