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Updated: Mar 21, 2026

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices
Published on: November 5, 2016
Reducing Escherichia coli growth on a composite biomaterial by a surface immobilized antimicrobial peptide
Gavin A Buckholtz1, Nina A Reger1, William D Anderton2
1Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, PA 15282, USA.
Abstract:
A new composite bioceramic consisting of calcium aluminum oxide (CaAlO) and hydroxyapatite (HA) was functionalized with the synthetic antimicrobial peptide Inverso-CysHHC10. CaAlO is a bioceramic that can be mold cast easily and quickly at room temperature. Improved functionality was previously achieved through surface reactions. Here, composites containing 0-5% HA (by mass) were prepared and the elastic modulus and modulus of rupture were mechanically similar to non-load bearing bone. The addition of hydroxyapatite resulted in increased osteoblast attachment (>180%) and proliferation (>140%) on all composites compared to 100% CaAlO. Antimicrobial peptide (AMP) immobilization was achieved using an interfacial alkene-thiol click reaction. The linked AMP persisted on the composite (>99.6% after 24h) and retained its activity against Escherichia coli based on N-phenylnaphthylamine uptake and bacterial turbidity tests. Overall, this simple scaffold system improves osteoblast activity and reduces bacterial activity.
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