Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Mar 21, 2026

ROS Live Cell Imaging During Neuronal Development

ROS Live Cell Imaging During Neuronal Development

Published on: February 9, 2021

Hydrogen peroxide (H2O2) controls axon pathfinding during zebrafish development.

Carole Gauron¹, Francesca Meda², Edmond Dupont¹

¹Centre Interdisciplinaire de Recherche en Biologie (CIRB), CNRS UMR 7241/INSERM U1050/Collège de France, 11, Place Marcelin Berthelot, 75231 Paris Cedex 05, France; PSL Research University, 75005 Paris, France.

Developmental Biology

|May 10, 2016

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CD133 Shapes Extracellular Vesicle Cargo and Angiogenic Function in Basal-Like Triple-Negative Breast Cancer.

Journal of extracellular vesicles·2026

Same author

IL-22 from enteroendocrine cells promotes early-life gut motility in zebrafish through the microbiota.

Science (New York, N.Y.)·2026

Same author

Professional identity work in emerging healthcare professions: current perspectives, gaps, and future directions.

Frontiers in health services·2026

Same author

Two-photon voltage imaging with rhodopsin-based sensors.

Neuron·2026

Same author

Author Correction: Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1.

The EMBO journal·2026

Same author

Bi-allelic loss-of-function variants in JKAMP cause a neurodevelopmental syndrome associated with dysregulation of GPR37 trafficking.

American journal of human genetics·2026

This summary is machine-generated.

Hydrogen peroxide (H2O2) plays a dynamic role in zebrafish embryonic development, particularly in brain and axonal guidance. This study reveals H2O2 regulates retinal ganglion cell axonal growth via the Hedgehog pathway.

Area of Science:

Developmental Biology
Cellular Biology
Neuroscience

Background:

Hydrogen peroxide (H2O2) is increasingly recognized for its physiological roles.
Its specific functions and distribution during embryonic development remain largely unknown.

Purpose of the Study:

To investigate the spatio-temporal dynamics and functions of H2O2 during zebrafish embryonic development.
To elucidate the role of H2O2 in brain development and axonal guidance.

Main Methods:

Utilized a genetic sensor to map H2O2 levels during zebrafish morphogenesis.
Employed biochemical and pharmacological approaches to study H2O2 distribution and degradation.
Investigated the impact of H2O2 modulation on retinal ganglion cell (RGC) axonal projections and the Hedgehog pathway.

Keywords:

Axon pathfinding Hedgehog Hydrogen peroxide

More Related Videos

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Published on: February 16, 2009

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Published on: October 27, 2023

Related Experiment Videos

Last Updated: Mar 21, 2026

ROS Live Cell Imaging During Neuronal Development

ROS Live Cell Imaging During Neuronal Development

Published on: February 9, 2021

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Published on: February 16, 2009

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Published on: October 27, 2023

Main Results:

Revealed dynamic spatio-temporal patterns of H2O2 during zebrafish development, with peak levels during organogenesis.
Demonstrated H2O2 enrichment in the developing brain and its critical role in axonal guidance.
Showed that H2O2 depletion impairs RGC axonal projections, a defect rescued by H2O2 or Hedgehog pathway activation.
Confirmed H2O2 directly modulates Hedgehog secretion ex vivo.

Conclusions:

Physiological H2O2 levels are crucial for zebrafish embryonic development, particularly for brain morphogenesis and RGC axonal growth.
H2O2 regulates axonal growth by modulating the Hedgehog pathway.
Enzymatic degradation is a key factor controlling H2O2 distribution during development.