Detection of 1,N(2)-propano-2'-deoxyguanosine in human urine by stable isotope dilution UHPLC-MS/MS analysis
Ning Zhang1, Yuanyuan Song2, Weibing Zhang3
1Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Abstract:
A sensitive and accurate stable isotope dilution UHPLC-MS/MS method was developed and validated for the detection and quantification of ProdG adducts in human urine, a surrogate for the ProdG adducts in genomic DNA of human. A specific solid phase extraction (SPE) approach was established for selective enrichment of urinary ProdG adducts and elimination of urinary matrix facilitating the coupled MS/MS detection. The recovery of the method is estimated about 84.8-107.2%, and the precision are about 0.8-3.6% for intraday and 2.8-10.0% for interday. Due to that the matrix effect is efficiently eliminated by SPE pretreatment, the limits of detection (LODs, S/N=3) and quantification (LOQs, S/N=10) are decreased to 100 and 300 amol for urinary ProdG adducts, respectively. By coupling the developed SPE pretreatment with the UHPLC-MS/MS analysis, ProdG adducts were accurately quantified in healthy human urine.
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