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Downregulation of the long non-coding RNA TUSC7 promotes NSCLC cell proliferation and correlates with poor prognosis
Zhongwei Wang1, Yingying Jin1, Hongtao Ren1
1Department of Medical Oncology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University Xi'an 710004, China.
Background:
Emerging evidence indicated that dysregulated long non-coding RNAs (lncRNAs) was implicated in the tumorigenesis and progression. LncRNA TUSC7 was involved in various malignancies. However, the role of TUSC7 in human non-small-cell lung cancer (NSCLC) remains unclear.
Methods:
Expression of TUSC7 was analyzed in 112 cases of NSCLC tissues and six lung cancer cell lines by quantitative real-time PCR (qRT-PCR). Then the correlation of TUSC7 expression with clinicopathological features and prognosis was aslo studied. Furthermore, overexpression of TUSC7 was performed and its role in tumor progression was explored.
Results:
The expression level of TUSC7 was lower in NSCLC tissues and lung cancer cells compared with their normal counterparts. Lower expression of TUSC7 in NSCLC tissues was associated with larger tumor size and higher TNM stage. Patients with lower TUSC7 expression had worse overall survival compared with the high expression cases. Univariate and multivariate analyses suggested that low expression of TUSC7 was an independent poor prognostic indicator for NSCLC patients. Moreover, upregulation of TUSC7 expression could inhibit proliferation of lung cancer cell in vitro.
Conclusions:
Our results suggested that TUSC7 was a potential biomarker for NSCLC prognosis, and the dysregulation of TUSC7 may play an important role in the NSCLC progression.
Insights
Long non-coding RNA TUSC7 (TUSC7) is downregulated in non-small cell lung cancer (NSCLC), correlating with poor prognosis. Upregulating TUSC7 inhibits lung cancer cell proliferation, suggesting its potential as a biomarker.
Area of Science:
- Molecular oncology
- Gene expression regulation
- Cancer biomarkers
Background:
- Dysregulated long non-coding RNAs (lncRNAs) are implicated in tumorigenesis.
- LncRNA TUSC7 is involved in various malignancies, but its role in non-small cell lung cancer (NSCLC) is unclear.
Purpose of the Study:
- To investigate the expression and function of TUSC7 in human NSCLC.
- To evaluate TUSC7 as a potential prognostic biomarker for NSCLC patients.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to analyze TUSC7 expression in 112 NSCLC tissues and cell lines.
- Correlation analysis of TUSC7 expression with clinicopathological features and patient prognosis.
- In vitro experiments involving TUSC7 overexpression to assess its effect on lung cancer cell proliferation.
Main Results:
- TUSC7 expression was significantly lower in NSCLC tissues and cells compared to normal counterparts.
- Lower TUSC7 expression correlated with larger tumor size, advanced TNM stage, and worse overall survival.
- Low TUSC7 expression was identified as an independent poor prognostic indicator for NSCLC.
- Upregulation of TUSC7 inhibited lung cancer cell proliferation in vitro.
Conclusions:
- TUSC7 is a potential prognostic biomarker for NSCLC.
- TUSC7 dysregulation plays a role in NSCLC progression.
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