Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulatory and Ethical Frameworks for First-in-Human Clinical Trials in India: Proceedings of a Codesigned Capacity-Building Workshop.

Clinical drug investigation·2026
Same author

Safety and efficacy of combined portal and hepatic vein embolisation in patients with colorectal liver metastases (DRAGON1): a multicentre, single-arm clinical trial.

The Lancet regional health. Europe·2025
Same author

The safety of telemedicine clinics as an alternative to in-person preoperative assessment for elective laparoscopic cholecystectomy in patients with benign gallbladder disease: a retrospective cohort study.

Patient safety in surgery·2023
Same author

Living donor uterus transplant in the UK: A case report.

BJOG : an international journal of obstetrics and gynaecology·2023
Same author

Donor-derived Epstein-Barr Virus Mucocutaneous Ulceration: A Unique and Complex Case of Rectal Hemorrhage.

Transplantation direct·2023
Same author

Autologous blood transfusion reduces the requirement for perioperative allogenic blood transfusion in patients undergoing major hepatopancreatobiliary surgery: a retrospective cohort study.

International journal of surgery (London, England)·2023

Related Experiment Video

Updated: Mar 21, 2026

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

18.0K

A simple and effective heat induced antigen retrieval method.

Vinod K R1, Denny Jones1, Venkatesha Udupa1

  • 1Department of Toxicology, Glenmark Research Centre, Navi Mumbai 400709, India.

Methodsx
|May 10, 2016
PubMed
Summary

This study introduces a simple paperclip method to prevent tissue damage during heat-induced antigen retrieval. This technique enhances antibody staining quality for formalin-fixed paraffin-embedded tissues, offering a safe and economical alternative.

Keywords:
Antigen retrievalImmunostainingModified HIER methodNew methodTissue damage

More Related Videos

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
09:38

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

Published on: August 30, 2013

13.1K
Author Spotlight: Effective Immunofluorescent Staining Protocol for Intestinal Cell Biology Research
08:25

Author Spotlight: Effective Immunofluorescent Staining Protocol for Intestinal Cell Biology Research

Published on: July 19, 2024

7.8K

Related Experiment Videos

Last Updated: Mar 21, 2026

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

18.0K
Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
09:38

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

Published on: August 30, 2013

13.1K
Author Spotlight: Effective Immunofluorescent Staining Protocol for Intestinal Cell Biology Research
08:25

Author Spotlight: Effective Immunofluorescent Staining Protocol for Intestinal Cell Biology Research

Published on: July 19, 2024

7.8K

Area of Science:

  • Histopathology
  • Immunohistochemistry
  • Biomedical Research

Background:

  • Heat-induced antigen retrieval is crucial for antibody staining in formalin-fixed paraffin-embedded (FFPE) tissues.
  • Standard methods can cause tissue damage or loss, compromising staining quality.
  • Existing retrieval methods may involve expensive or complex equipment like microwaves or pressure cookers.

Purpose of the Study:

  • To describe an improved, simple method for heat-induced antigen retrieval.
  • To enhance the preservation and quality of FFPE tissue sections during epitope retrieval.
  • To provide a safer, more economical, and efficient alternative to current antigen retrieval techniques.

Main Methods:

  • A modified heat-induced antigen retrieval protocol was developed.
  • The modification involves overlapping a tissue slide with a plain slide, secured by a paperclip with a minimal gap.
  • Tissues were then subjected to standard heat treatment in a buffer solution.

Main Results:

  • The modified method successfully prevented tissue damage, folding, and detachment during heating.
  • Tissues remained intact even at high temperatures, leading to improved antibody staining quality.
  • The technique proved to be safe, economical, effective, and less time-consuming than conventional methods.

Conclusions:

  • The simple paperclip-assisted overlapping slide method is a valuable improvement for heat-induced antigen retrieval.
  • This technique enhances tissue integrity and staining quality in FFPE sections.
  • It offers a practical, cost-effective, and efficient solution for immunohistochemistry laboratories.