Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gastrulation01:56

Gastrulation

68.7K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
68.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The zebrafish as a model to study polycystic liver disease.

Zebrafish·2013
Same author

α-actinin is required for the proper assembly of Z-disk/focal-adhesion-like structures and for efficient locomotion in Caenorhabditis elegans.

Journal of molecular biology·2010
Same author

Nicotine response genetics in the zebrafish.

Proceedings of the National Academy of Sciences of the United States of America·2009
Same author

An intracellular serpin regulates necrosis by inhibiting the induction and sequelae of lysosomal injury.

Cell·2007

Related Experiment Video

Updated: Mar 21, 2026

Behavioral Tracking and Neuromast Imaging of Mexican Cavefish
14:58

Behavioral Tracking and Neuromast Imaging of Mexican Cavefish

Published on: April 6, 2019

8.4K

Hatching Fish-When Should Animal Tracking Begin?

Gary L Moulder1

  • 1CVRI Zebrafish Shared Facility , UCSF, San Francisco, California.

Zebrafish
|May 10, 2016
PubMed
Summary
This summary is machine-generated.

The National Institutes of Health (NIH) now requires animal oversight for zebrafish at 3 days postfertilization (dpf), differing from the traditional 7 dpf. This policy impacts research by necessitating the accounting of all zebrafish embryos and larvae.

More Related Videos

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish
14:03

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

11.5K
Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish
10:56

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Published on: March 6, 2014

13.1K

Related Experiment Videos

Last Updated: Mar 21, 2026

Behavioral Tracking and Neuromast Imaging of Mexican Cavefish
14:58

Behavioral Tracking and Neuromast Imaging of Mexican Cavefish

Published on: April 6, 2019

8.4K
Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish
14:03

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

11.5K
Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish
10:56

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Published on: March 6, 2014

13.1K

Area of Science:

  • Animal research ethics
  • Zebrafish developmental biology

Background:

  • The National Institutes of Health (NIH) Office of Laboratory Animal Welfare (OLAW) issued a policy in 2009 regarding zebrafish euthanasia.
  • This policy established a new developmental stage for OLAW oversight based on avian hatching timelines.

Purpose of the Study:

  • To provide context and real-world experience regarding the NIH-OLAW zebrafish policy.
  • To initiate a community discussion on the appropriateness of the 3 days postfertilization (dpf) hatching time point for policy establishment.

Main Methods:

  • Review and synthesis of practical experiences with the OLAW zebrafish policy.
  • Comparison of the established 3 dpf policy with the traditional 7 dpf community standard.

Main Results:

  • The 2009 OLAW policy sets the oversight threshold for zebrafish at 3 dpf, contrasting with the long-standing community practice of 7 dpf.
  • This policy change has significant implications, requiring researchers to account for all zebrafish embryos and larvae.

Conclusions:

  • The current 3 dpf policy presents challenges and necessitates careful consideration.
  • Further community dialogue is needed to evaluate the 3 dpf hatching time point as the definitive policy standard for zebrafish research oversight.