A bacterial phytochrome-based optogenetic system controllable with near-infrared light

Area of Science:

• Optogenetics
• Molecular Biology
• Biochemistry

Background:

• Optogenetics enables light-mediated control of cellular pathways.
• Bacterial phytochromes offer potential for novel optogenetic tools.

Purpose of the Study:

• To develop and characterize a new optogenetic system based on the BphP1-PpsR2 interaction.
• To demonstrate light-inducible control of protein localization and gene expression.

Main Methods:

• In vitro and mammalian cell characterization of BphP1-PpsR2 interaction.
• Protein translocation to cellular compartments (plasma membrane, nucleus).
• Assessment of light-dependent gene expression in vitro and in vivo.

Main Results:

• Demonstrated reversible light-induced binding between BphP1 and PpsR2.
• Achieved light-inducible control of cell morphology and protein localization.
• Showcased significant light-dependent gene expression contrast (40-fold in vitro, 32-fold in vivo).

Conclusions:

• The BphP1-PpsR2 system provides a sensitive optogenetic tool.
• Near-infrared light sensitivity and endogenous chromophore utilization enhance applicability.
• Spectral compatibility allows for multi-color optogenetics.