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Updated: Mar 21, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
Published on: May 23, 2025
Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes
David W Morgens1, Richard M Deans1,2, Amy Li1
1Department of Genetics, Stanford University, Stanford, California, USA.
Abstract:
We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We found that the precision of the two libraries in detecting essential genes was similar and that combining data from both screens improved performance. Notably, results from the two screens showed little correlation, which can be partially explained by the identification of distinct essential biological processes with each technology.
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