Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Probable essential thrombocythemia in a dog.

P E Hopper1, C P Mandell, J M Turrel

  • 1Small Animal Internal Medicine Service, Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis.

Journal of Veterinary Internal Medicine
|April 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolation of bluetongue virus from Culicoides sp. in India.

Revue scientifique et technique (International Office of Epizootics)·2020
Same author

Platelet Activation and Clopidogrel Effects on ADP-Induced Platelet Activation in Cats with or without the A31P Mutation in MYBPC3.

Journal of veterinary internal medicine·2016
Same author

Impact factor: Is it the ultimate parameter for the quality of publication?

Indian journal of medical microbiology·2016
Same author

Association of Factor V Secretion with Protein Kinase B Signaling in Platelets from Horses with Atypical Equine Thrombasthenia.

Journal of veterinary internal medicine·2015
Same author

Predatory journals.

Indian journal of medical microbiology·2015
Same author

Information retrieval of tuberculosis literature in e-databases.

The Indian journal of tuberculosis·2014

This report describes a rare case of a blood disorder called essential thrombocythemia in an elderly dog. The animal showed extremely high platelet counts, bone marrow changes, and signs of organ enlargement. Clinicians used specialized staining and imaging to confirm the diagnosis. Treatment with radioactive phosphorus successfully lowered blood cell counts, though the patient later succumbed to unrelated health complications.

Area of Science:

  • Veterinary pathology and essential thrombocythemia diagnostics
  • Hematology and oncology research within clinical veterinary medicine

Background:

Veterinary clinicians often struggle to identify rare myeloproliferative disorders in aging canine patients. Prior research has shown that persistent elevation of blood platelets frequently stems from secondary inflammatory or neoplastic processes. No prior work had resolved the diagnostic criteria for primary bone marrow conditions in dogs exhibiting extreme thrombocytosis. That uncertainty drove the need for detailed case documentation to improve clinical recognition. It was already known that human diagnostic standards for similar blood conditions rely on specific marrow morphology. This gap motivated the current investigation into canine hematological abnormalities. Researchers previously lacked comprehensive data linking clinical symptoms like gastrointestinal bleeding to these specific marrow findings. The present report addresses this deficiency by detailing a unique case of persistent platelet elevation.

Purpose Of The Study:

The aim of this study is to document a rare case of essential thrombocythemia in an elderly dog. This report addresses the diagnostic challenges associated with distinguishing primary blood disorders from secondary reactive conditions. Researchers sought to characterize the specific hematological and marrow findings that define this myeloproliferative state. The investigation focuses on the utility of cytochemical and ultrastructural analysis in confirming the diagnosis. Clinicians were motivated by the need to provide a detailed account of successful therapeutic intervention using radiophosphorus. This work explores the relationship between persistent thrombocytosis and marrow megakaryocytic hyperplasia. The authors intend to provide a reference for veterinary practitioners encountering similar clinical presentations. This study serves to clarify the pathological features of this rare condition in canine medicine.

Keywords:
canine hematologymegakaryocytic hyperplasiathrombocytosis diagnosisveterinary pathology

Frequently Asked Questions

The researchers propose that the condition is a myeloproliferative disease characterized by persistent thrombocytosis, abnormal platelet morphology, and marked megakaryocytic hyperplasia. This diagnosis relies on excluding reactive causes while identifying specific quantitative and qualitative changes within the bone marrow series.

Clinicians utilized a positive acetylcholinesterase reaction to confirm the presence of megakaryocytes and megakaryoblasts. This specific cytochemical tool allows for the identification of the megakaryocytic lineage within the bone marrow samples.

The authors state that radiophosphorus was necessary to achieve a rapid decline in platelet, megakaryocyte, and basophil counts. This therapeutic intervention targeted the hyperplastic marrow activity observed in the patient.

The study utilized cytochemical and ultrastructural data to differentiate the primary condition from secondary reactive thrombocytosis. These findings provided the necessary evidence to support the diagnosis of essential thrombocythemia.

Related Experiment Videos

Main Methods:

The review approach involved a comprehensive analysis of clinical, laboratory, and pathological data from an eleven-year-old canine patient. Investigators performed serial blood counts to monitor the persistent elevation of platelets over time. Bone marrow aspiration provided samples for morphological evaluation and specialized staining techniques. The team employed an acetylcholinesterase reaction to verify the presence of specific megakaryocytic cell lines. Ultrastructural examination of the marrow tissue allowed for detailed assessment of cellular abnormalities. Clinicians documented the history of splenomegaly and gastrointestinal bleeding to establish the full clinical picture. The therapeutic strategy utilized radiophosphorus administration to manage the excessive blood cell production. Researchers tracked the response to this treatment by measuring changes in peripheral blood and marrow cell populations.

Main Results:

Key findings from the literature indicate that the dog exhibited platelet counts ranging from 4.19 to 4.95 million per microliter. The bone marrow analysis revealed marked megakaryocytic hyperplasia alongside an absence of circulating megakaryoblasts. Researchers observed that 15% to 20% of the marrow cells were megakaryoblasts and megakaryocytes. A positive acetylcholinesterase reaction confirmed the identity of these proliferating cells. The patient also displayed a significant basophilia count of 4,836 per microliter in both blood and marrow samples. Treatment with radiophosphorus resulted in a rapid decline of megakaryoblasts, megakaryocytes, platelets, and basophils. These hematological improvements occurred before the patient succumbed to unrelated acute necrotizing pancreatitis and diabetes mellitus. The data demonstrate that the clinical features were consistent with a primary myeloproliferative disorder rather than reactive thrombocytosis.

Conclusions:

The authors propose that the observed clinical and laboratory features align with a myeloproliferative disease diagnosis. This synthesis suggests that cytochemical staining provides a reliable method for identifying megakaryocytic lineage expansion. The findings imply that radiophosphorus therapy effectively reduces excessive platelet and megakaryocyte production in canine patients. These results indicate that rapid hematological improvement is achievable even in advanced cases of the disorder. The authors note that the patient death resulted from unrelated systemic complications rather than the primary blood condition. This review implies that clinicians should consider this diagnosis when reactive causes for thrombocytosis are excluded. The evidence supports the use of specialized ultrastructural analysis to confirm suspected marrow abnormalities. Future clinical management should prioritize monitoring for secondary metabolic issues during therapy.

The patient exhibited a basophilia count of 4,836 per microliter in the blood and bone marrow. This measurement served as a significant indicator of the underlying myeloproliferative process.

The researchers propose that the patient death was caused by acute necrotizing pancreatitis and diabetes mellitus. These metabolic complications occurred unexpectedly before the dog reached a state of complete remission.