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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Related Experiment Video

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Advancements in the Metabolic Profiling of Three-Dimensional Brain Tumor Spheroids for Drug Screening
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Engineered bacteria detect spatial profiles in glucose concentration within solid tumor cell masses.

Jan T Panteli1, Neil S Forbes2

  • 1Department of Chemical Engineering, University of Massachusetts, 686 North Pleasant Street, Amherst, Massachusetts, 01003.

Biotechnology and Bioengineering
|May 10, 2016
PubMed
Summary
This summary is machine-generated.

Engineered bacteria sense glucose gradients in tumors, enabling targeted drug delivery. This approach promises improved cancer treatment by reaching deeper tumor regions and adapting dosage to metabolic activity.

Keywords:
E. coliTrz1bacterial cancer therapybiosensorcancer

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Area of Science:

  • Biotechnology
  • Cancer Research
  • Microbiology

Background:

  • Tumor heterogeneity complicates cancer treatment.
  • Conventional drugs struggle to penetrate deep into tumors.
  • Targeting both fast-growing and slow-growing tumor regions is crucial.

Purpose of the Study:

  • To engineer glucose-sensing bacteria for targeted cancer therapy.
  • To investigate bacteria's ability to detect glucose gradients within tumors.
  • To evaluate bacteria as a drug delivery system for improved tumor penetration and nutrient-dependent dosing.

Main Methods:

  • Designed a genetic circuit in E. coli using Trz1-GFP to sense glucose and ribose.
  • Utilized a microfluidic tumor model simulating systemic delivery and clearance.
  • Measured GFP expression to indicate glucose concentration at different depths.

Main Results:

  • GFP expression correlated with glucose concentration and tumor cell viability.
  • Glucose concentration decreased with penetration depth.
  • Mathematical models predicted superior tumor cell elimination by glucose-sensing bacteria compared to conventional drugs.

Conclusions:

  • Glucose-sensing bacteria can identify and target metabolically active regions within tumors.
  • This engineered bacteria system offers enhanced drug penetration and nutrient-dependent dosing.
  • The approach holds potential for treating viable tumor regions prone to metastasis.