Reproducibility of the indirect immunobead assay for detecting sperm antibodies in serum

Although an immunobead assay (IBA) for the detection of antisperm antibodies was developed several years ago and has been used for the study of immunologic infertility, no data regarding its variability and reproducibility are yet available. We evaluated the intraassay reproducibility of the indirect IBA by testing aliquots of antisperm-antibody-positive sera from two patients against the same donor sperm sample. The interassay reproducibility was evaluated by testing a positive serum sample first with different sperm samples from the same donor and second with sperm samples from different donors. The results of those experiments showed that the indirect IBA has very low intraassay variation and greater interassay variability.