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Reduced miR-485-5p expression predicts poor prognosis in patients with gastric cancer
1Laboratory of Stem Cell Biology and Department of Pediatric Surgery, State Key Laboratory of Biotherapy and Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China. xianmin_h@163.com.
Objective:
The present study was designed to explore expression and prognostic value of miR-485-5p in patients with gastric cancer.
Patients And Methods:
We determined the expression level of miR-485-5p in 132 cases of paired GC and adjacent non-tumor tissues by quantitative real-time PCR (qRT-PCR). The relevance of miR-485-5p expression to the clinicopathological factors was assessed. Overall survival (OS) was examined using Kaplan-Meier curves and the Cox proportional hazards regression model.
Results:
The expression of miR-485-5p was significantly down-regulated in GC tissues compared with adja-cent normal tissues (p < 0.01). MiR-485-5p expression was positively correlated with larger tumor size (p = 0.003), deeper invasion depth, (p = 0.005), positive lymph node metastasis (p = 0.039), advanced tumor-node-metastasis (TNM) stage (p = 0.017). Patients survival analysis showed that a clear positive correlation between miR-485-5p expression level and survival time of gastric cancer patients (p < 0.001). Multivariate analyses confirmed that a low level of miR-485-5p expression was an independent predictor of poor prognosis in GC patients.
Conclusions:
Expression level of miR-485-5p serves as a novel biomarker for the overall survival of patients with gastric cancer.
