Related Experiment Video
Updated: Mar 21, 2026
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Published on: September 6, 2014
12.8K
Stem Cell Surface Marker Expression Defines Late Stages of Reprogramming to Pluripotency in Human Fibroblasts
Jordan E Pomeroy1, Shelley R Hough2, Kathryn C Davidson3
1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
Stem Cells Translational Medicine
|May 11, 2016
Summary
Researchers developed new tools to identify fully reprogrammed cells during the late stages of cellular reprogramming. This method uses cell surface markers like CDH3 to efficiently isolate induced pluripotent stem cells for research and therapy.
Area of Science:
- Cell Biology
- Stem Cell Biology
- Epigenetics
Background:
- Cellular reprogramming to pluripotency involves distinct stages with transcriptome and epigenome changes.
- The final phase of reprogramming remains poorly understood, hindering efficient derivation of pluripotent stem cells.
Purpose of the Study:
- To develop tools for characterizing the transition to a fully reprogrammed state.
- To identify markers for the late stages of cellular reprogramming.
Main Methods:
- Isolation of colonies from transfected human fibroblasts using stem cell surface markers.
- Analysis of pluripotency and germ lineage gene expression.
- Live-cell staining with GCTM-2 antibody and anti-CDH3.
Main Results:
- Expression of CDH3 and other pluripotency genes marks a late stage of reprogramming.
- Combined GCTM-2 and anti-CDH3 staining identified cells with embryonic stem cell-like gene expression.
- These identified cells efficiently formed stable induced pluripotent stem cell lines.
Conclusions:
- The study provides tools to better understand the final stages of human cell reprogramming.
- A simple method for prospective identification of fully reprogrammed cells is established.
- This facilitates research and enhances the efficiency of deriving cell lines for therapeutic applications.
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