Related Experiment Video

Updated: Jan 25, 2026

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction
09:28

Fabrication and Testing of Catalytic Aerogels Prepared Via Rapid Supercritical Extraction

Published on: August 31, 2018

7.7K

TET2 Regulates Mast Cell Differentiation and Proliferation through Catalytic and Non-catalytic Activities

Sara Montagner1, Cristina Leoni1, Stefan Emming1

  • 1Institute for Research in Biomedicine, Universita' della Svizzera italiana (USI), 6500 Bellinzona, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.

Cell Reports
|May 11, 2016
PubMed

Insights

TET2 dioxygenase is vital for mast cell development. Its absence disrupts gene expression and 5hmC patterns, impairing differentiation but boosting proliferation, which TET2 uniquely controls.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Immunology

Background:

  • TET family dioxygenases convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), influencing genome function.
  • 5hmC is a key epigenetic mark involved in gene regulation and cellular processes.

Purpose of the Study:

  • To investigate the role of TET2 in mast cell differentiation and proliferation.
  • To elucidate the mechanisms underlying TET2's function in mast cells.

Main Methods:

  • CRISPR-Cas9 mediated Tet2 ablation in mast cells.
  • Analysis of gene expression and genome-wide 5hmC distribution.
  • Modulation of other TET family members' activity.
  • Rescue experiments with TET2 re-expression.

Main Results:

  • Tet2 ablation in mast cells led to disrupted gene expression and altered 5hmC patterns, particularly at enhancers.
  • Impaired mast cell differentiation in Tet2-ablated cells was partially rescued by other TET family members and linked to C/EBP transcription factors.
  • Mast cell proliferation significantly increased upon Tet2 loss, a phenotype exclusively rescued by TET2 re-expression, irrespective of its catalytic activity.

Conclusions:

  • Mast cell differentiation relies on compensatory mechanisms involving other TET family members when TET2 is absent.
  • Mast cell proliferation is strictly dependent on TET2 expression, highlighting a unique role for TET2 beyond its catalytic activity.
Keywords:
DNA hydroxymethylationTETdifferentiationepigeneticsmast cellsproliferation

More Related Videos

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays09:36

Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

Published on: August 13, 2017

7.1K
Preparation and 3D Tracking of Catalytic Swimming Devices06:50

Preparation and 3D Tracking of Catalytic Swimming Devices

Published on: July 1, 2016

7.9K

Related Concept Videos

Turnover Number and Catalytic Efficiency01:19

Turnover Number and Catalytic Efficiency

The turnover number of an enzyme is the maximum number of substrate molecules it can transform per unit time. Turnover numbers for most enzymes range from 1 to 1000 molecules per second. Catalase has the known highest turnover number, capable of converting up to 2.8×106 molecules of hydrogen peroxide into water and oxygen per second. Lysozyme has the lowest known turnover number of half a molecule per second.
Chymotrypsin is a pancreatic enzyme that breaks down proteins during digestion....
20.4K
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
5.0K
Reduction of Benzene to Cyclohexane: Catalytic Hydrogenation01:28

Reduction of Benzene to Cyclohexane: Catalytic Hydrogenation

Unlike the easy catalytic hydrogenation of an alkene double bond, hydrogenation of a benzene double bond under similar reaction conditions does not take place easily. For example, in the reduction of stilbene, the benzene ring remains unaffected while the alkene bond gets reduced. Hydrogenation of an alkene double bond is exothermic and a favorable process. In contrast, to hydrogenate the first unsaturated bond of benzene, an energy input is needed; that is, the process is endothermic. This is...
5.8K
Reduction of Alkenes: Catalytic Hydrogenation02:13

Reduction of Alkenes: Catalytic Hydrogenation

Alkenes undergo reduction by the addition of molecular hydrogen to give alkanes. Because the process generally occurs in the presence of a transition-metal catalyst, the reaction is called catalytic hydrogenation.
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
14.0K
Reduction of Alkynes to cis-Alkenes: Catalytic Hydrogenation02:24

Reduction of Alkynes to cis-Alkenes: Catalytic Hydrogenation

Introduction
Like alkenes, alkynes can be reduced to alkanes in the presence of transition metal catalysts such as Pt, Pd, or Ni. The reaction involves two sequential syn additions of hydrogen via a cis-alkene intermediate.
9.0K
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.8K