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Published on: July 20, 2016
[ATP and ADP of platelets in chronic myeloproliferative disorders and multiple myeloma]
Abstract:
The content of platelet adenine nucleotide in chronic myeloproliferative disorders (CMPD) and multiple myeloma (MM) was measured by a luciferin-luciferase method by Holmsen and Weiss. The release of ATP and ADP from platelet during aggregation induced by collagen and epinephrine were analyzed. The total 42 investigated cases consisted of 11 cases of polycythemia vera (PV), 7 cases of essential thrombocythemia (ET), 7 cases of chronic myeloid leukemia (CML), 9 cases of blastic crisis of CML (BC-CML), and 8 cases of multiple myeloma (MM). The healthy control was 19 cases. In CMPD and MM, the amount of ATP was normal in spite of decrease of ADP; therefore, the ratio of ATP/ADP increased. On the other hand, the ATP significantly increased in BC-CML. MM revealed a remarkable increase of ATP release due to the aggregation by collagen and epinephrine. The maximal rate of aggregation of collagen and epinephrine using Lumi-aggregometer indicated a positive relationship with the ATP release by the Holmsen and Weiss' method. The platelet volume in CMPD increased showing correlation with ATP content and not with ADP. In conclusion, CMPD and MM are regarded as acquired qualitative disorders of platelets or secondary storage pool diseases from the view points of the abnormalities in ATP, ADP contents and their release. However, BC-CML and MM revealed some different change from that of CMPD.
Insights
Platelet adenine nucleotide levels are altered in chronic myeloproliferative disorders (CMPD) and multiple myeloma (MM), indicating acquired qualitative platelet disorders. Blastic crisis of CML (BC-CML) showed distinct ATP changes.
Area of Science:
- Hematology
- Biochemistry
- Oncology
Context:
- Platelet adenine nucleotides (ATP and ADP) play crucial roles in platelet function and aggregation.
- Chronic myeloproliferative disorders (CMPD) and multiple myeloma (MM) are hematological malignancies with potential effects on platelet physiology.
- Understanding platelet adenine nucleotide dynamics is important for characterizing these conditions.
Purpose:
- To investigate and compare platelet adenine nucleotide content and release in patients with CMPD, multiple myeloma (MM), and healthy controls.
- To analyze the relationship between platelet aggregation and adenine nucleotide release.
- To explore the potential of these biochemical markers in classifying hematological disorders.
Summary:
- Platelet ATP and ADP levels were measured in 42 patients (polycythemia vera, essential thrombocythemia, chronic myeloid leukemia, blastic crisis of CML, MM) and 19 controls.
- CMPD and MM showed normal ATP with decreased ADP, increasing the ATP/ADP ratio. Blastic crisis of CML exhibited significantly increased ATP.
- Multiple myeloma demonstrated increased ATP release upon aggregation, correlating with aggregation rates. Platelet volume correlated with ATP in CMPD.
Impact:
- Findings suggest CMPD and MM represent acquired qualitative platelet disorders or secondary storage pool diseases due to altered adenine nucleotide metabolism.
- Blastic crisis of CML and MM exhibit unique biochemical profiles compared to other CMPD.
- This research contributes to understanding platelet dysfunction in hematological malignancies and may inform diagnostic or therapeutic strategies.
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