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Highly sensitive nanoparticle-based immunoassays with elemental detection: Application to Prostate-Specific Antigen

Marta Garcia-Cortes¹, Jorge Ruiz Encinar¹, Jose M Costa-Fernandez¹

¹Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, Oviedo 33006, Spain.

Biosensors & Bioelectronics

|May 11, 2016

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A novel immunoassay platform using Mn-doped ZnS quantum dots enables highly sensitive cancer biomarker detection. This method achieves attogram-per-milliliter sensitivity for Prostate-Specific Antigen (PSA) detection.

Area of Science:

Biochemistry
Nanotechnology
Analytical Chemistry

Background:

Developing sensitive assay methods for biomolecules is challenging due to ultralow clinical sample concentrations.
High sensitivity is crucial for accurate detection of cancer biomarkers.

Purpose of the Study:

To present a Mn-doped ZnS quantum dots-based immunoassay platform for highly sensitive cancer biomarker detection.
To achieve ultrahigh sensitivity through gold nanoparticle amplification for enhanced detection.

Main Methods:

Utilized Mn-doped ZnS quantum dots as nanoparticle tags.
Employed gold deposition on nanoparticle tags as catalytic seeds for size amplification.
Measured gold content using elemental mass spectrometry for quantification.

Keywords:

Biomarkers detection Immunoassays Mass spectrometry Nanotechnology Quantum dots

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Main Results:

Achieved attogram-per-milliliter level detection of Prostate-Specific Antigen (PSA).
Demonstrated a broad dynamic range exceeding 8 orders of magnitude for PSA detection.
Successfully discriminated small differences in PSA concentrations in real serum samples.

Conclusions:

The developed immunoassay platform offers ultrahigh sensitivity and a broad dynamic range for biomarker detection.
This method is highly applicable for clinical studies requiring quantitative detection of diverse biomarkers at varying concentrations.
The platform shows promise for sensitive and reliable cancer biomarker analysis in clinical settings.