Related Experiment Video
Updated: Mar 21, 2026
Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance
Published on: June 14, 2024
Detection of canine visceral leishmaniasis by conjunctival swab PCR
Vanessa Figueredo Pereira1, Julia Cristina Benassi2, Wilma Aparecida Starke-Buzetti3
1Programa de Pós-Graduação em Epidemiologia Experimental Aplicada às Zoonoses, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
Introduction:
Conjunctival swab PCR was evaluated as a tool to diagnose visceral leishmaniasis in dogs.
Methods:
Conjunctival swab PCR was compared to indirect immunofluorescence antibody test and blood PCR.
Results:
Indirect immunofluorescence was significantly correlated with conjunctival swab PCR (p < 0.05), but not with blood PCR (p > 0.05). In addition, conjunctival swab PCR was significantly associated with presence of clinical symptoms (p < 0.05), whereas blood PCR was associated with absence of clinical symptoms (p < 0.05).
Conclusions:
Results indicate that conjunctival swab PCR is useful in epidemiological surveys of canine visceral leishmaniasis.
More Related Videos
07:40Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
Published on: June 29, 2020
08:10Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
Published on: May 2, 2019