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ABC-B transporter genes in Dirofilaria immitis
Catherine Bourguinat1, Hua Che1, Thangadurai Mani1
1Institute of Parasitology, McGill University, 21 111 Lakeshore Road, Sainte-Anne-de-Bellevue, H9X3V9, QC, Canada.
Summary
Researchers identified nine ABC transporter genes in Dirofilaria immitis, including three P-glycoproteins, potentially linked to macrocyclic lactone resistance. This discovery aids in understanding heartworm molecular biology and resistance mechanisms.
Area of Science:
- Molecular Biology
- Parasitology
- Genomics
Background:
- Dirofilaria immitis causes heartworm disease in canids, cats, and humans.
- Macrocyclic lactones (ML) are used for prevention, but resistance is emerging.
- ABC-B transporter genes are implicated in ML resistance in other nematodes.
Purpose of the Study:
- To identify all ABC-B transporter genes in Dirofilaria immitis.
- To investigate the potential role of these genes in ML resistance.
- To establish a foundation for future research on heartworm resistance mechanisms.
Main Methods:
- Bioinformatic analysis of the Dirofilaria immitis genome.
- PCR amplification of genomic DNA and cDNA.
- Phylogenetic analysis of identified ABC transporter genes.
Main Results:
- Nine ABC transporter genes, including one pseudogene, were identified in D. immitis.
- Three P-glycoproteins (Dim-pgp-3, Dim-pgp-10, Dim-pgp-11) were characterized.
- The number of ABC-B transporter genes is lower than in C. elegans and H. contortus.
Conclusions:
- The identified ABC transporters provide a basis for investigating ML resistance in D. immitis.
- Further studies are required to elucidate the functional role of these transporters in resistance.
- This research advances the understanding of the molecular biology of Dirofilaria immitis.
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