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Updated: Mar 21, 2026
A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy
Method-dependent Discrepancies in Fetal Hemoglobin Quantification in Patients With Hemoglobin S
Susan E Creary1, Amy L Pyle-Eilola, Elizabeth Varga
1*Division of Pediatric Hematology/Oncology/BMT †Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital ‡Department of Pathology, Ohio State University Wexner Medical Center, Columbus, OH §The Permanente Medical Group Regional Laboratories, Kaiser Permanente Northern California, Berkeley, CA ∥Department of Pathology and Laboratory Medicine, St Paul's Hospital ¶Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Hemoglobin F (HbF) concentration is used in the diagnosis of certain hemoglobinopathies and accurate quantification is central to treatment of patients with sickle cell disease. The 2 most commonly used methods to quantify HbF are high performance liquid chromatography and capillary zone electrophoresis. This study reports discrepancies in HbF quantification between these methods when hemoglobin S is present in the sample. Clinicians and investigators should be mindful of the method used for HbF quantification when evaluating and treating patients who produce hemoglobin S.
Hemoglobin F (HbF) concentration is used in the diagnosis of certain hemoglobinopathies and accurate quantification is central to treatment of patients with sickle cell disease. The 2 most commonly used methods to quantify HbF are high performance liquid chromatography and capillary zone electrophoresis. This study reports discrepancies in HbF quantification between these methods when hemoglobin S is present in the sample. Clinicians and investigators should be mindful of the method used for HbF quantification when evaluating and treating patients who produce hemoglobin S.
