Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

338
In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance...
338
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

295
Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
295
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

94
The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
94
One-Compartment Open Model: Urinary Excretion Data and Determination of k01:11

One-Compartment Open Model: Urinary Excretion Data and Determination of k

743
The one-compartment open model leverages urinary excretion data to estimate renal clearance, which gauges the kidney's capacity to expel a drug. This method offers several benefits, including directly measuring drug elimination and assessing the kidney's contribution to overall drug clearance. However, this approach has limitations. It assumes sole renal excretion of the drug, which is not true for all drugs. Accurate urinary excretion and plasma drug concentration measurement can also...
743
Factors Affecting Renal Clearance: Renal Impairment01:17

Factors Affecting Renal Clearance: Renal Impairment

548
Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
548
Drug Dosing in Renal Diseases: Dose Adjustments Based on Drug Clearance and Elimination Rate Constant01:25

Drug Dosing in Renal Diseases: Dose Adjustments Based on Drug Clearance and Elimination Rate Constant

299
In patients with renal disease, dosage adjustments are necessary to maintain therapeutic plasma drug concentrations and prevent toxicity or subtherapeutic exposure. Renal impairment alters drug pharmacokinetics, especially in conditions like uremia, where changes such as prolonged elimination half-life and altered apparent volume of distribution can significantly affect drug disposition. These changes require careful modification of the dosing regimen to achieve the desired clinical...
299

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factors affecting serum PFAS concentrations among US females with surgically and naturally induced menopause: data from NHANES 2003-2018.

Environmental science and pollution research international·2023
Same author

Co-variate adjusted associations between serum concentrations of selected perfluoroalkyl substances and urinary concentrations of selected arsenic species.

Environmental science and pollution research international·2022
Same author

Associations between concentrations of serum α-klotho and selected urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons: data for US adults aged 40-79 years.

Environmental science and pollution research international·2022
Same author

Serum concentrations of perfluoro-1-heptane sulfonate (PFHpS) among US adults: variabilities across different stages of kidney function.

Environmental science and pollution research international·2022
Same author

Associations of serum perfluoroalkyl substances with concentrations of blood manganese and selenium.

Environmental science and pollution research international·2022
Same author

Serum concentrations of selected perfluoroalkyl substances for US females compared to males as they age.

The Science of the total environment·2022

Related Experiment Video

Updated: Mar 21, 2026

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
09:09

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Published on: August 9, 2013

41.1K

Ratio-based vs. model-based methods to correct for urinary creatinine concentrations.

Ram B Jain1

  • 1, 2959 Estate View Court, Dacula, GA, USA. Jain.ram.b@gmail.com.

Environmental Science and Pollution Research International
|May 12, 2016
PubMed
Summary
This summary is machine-generated.

The ratio-based method for correcting urinary analyte concentrations is flawed as it only accounts for hydration. A model-based method, which considers factors like age, gender, and race/ethnicity, offers a more comprehensive approach to urinary creatinine concentration (UCR) correction.

Keywords:
AgeGenderHydrationRace/ethnicityUrinary creatinine correction

More Related Videos

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

44.4K
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.8K

Related Experiment Videos

Last Updated: Mar 21, 2026

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
09:09

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Published on: August 9, 2013

41.1K
A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

44.4K
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.8K

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Biostatistics

Background:

  • Urinary analyte concentration is typically corrected using the ratio of analyte to urinary creatinine concentration (UCR).
  • This ratio-based method assumes hydration is the sole determinant of UCR, neglecting other influencing factors.
  • Factors such as age, gender, and race/ethnicity are known to affect UCR, limiting the accuracy of the ratio-based method.

Purpose of the Study:

  • To evaluate and compare the performance of ratio-based and model-based creatinine correction methods.
  • To assess how gender, age, and race/ethnicity individually impact UCR correction methods for urinary analytes and metabolites.

Main Methods:

  • The study compared ratio-based correction with a proposed model-based correction method.
  • Model-based correction utilizes observed UCRs as independent variables in regression models.
  • The performance was evaluated by analyzing the effects of gender, age, and race/ethnicity on selected urinary analytes and metabolites.

Main Results:

  • The ratio-based method resulted in more statistically significant pairwise differences (e.g., between males and females, or different racial groups) compared to the model-based method.
  • However, the frequency of significant differences could vary depending on the specific analyte.
  • Comparisons of geometric mean ratios revealed differences in how each method estimated group differences, influenced by whether the UCR was higher or lower in the numerator group.

Conclusions:

  • The model-based creatinine correction method is recommended when accounting for all factors influencing UCR, including age, gender, and race/ethnicity.
  • The ratio-based method's limitations highlight the need for more sophisticated approaches in accurately assessing urinary analyte concentrations.
  • Accurate UCR correction is crucial for reliable interpretation of urinary analyte and metabolite levels in diverse populations.